Differential Metal Selectivity and Gene Expression of Two Zinc Transporters from Rice

Sunita A. Ramesh, Ryoung Shin, David J. Eide, Daniel P. Schachtman

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

Zinc is an essential mineral for a wide variety of physiological and biochemical processes. To understand zinc transport in cereals, we identified putative zinc transporters in gene databases. Three full-length cDNAs were identified and characterized from rice (Oryza sativa). Two of the cDNAs partially complemented a yeast (Saccharomyces cerevisiae) mutant deficient in zinc uptake at low concentrations. The two transporters showed many similarities in function but differed in ionic selectivity and pH optimum of activity. Expression patterns also differed between the two genes. One gene was broadly expressed under all conditions, and the other gene was mainly induced by zinc deficiency to higher levels in roots than in leaves. Although the timing of expression differed between the two genes, localization of expression overlapped in roots. Comparisons of the protein sequences, ionic selectivity, and gene expression patterns of the two transporters suggest that they may play different roles in the physiology of the whole plant.

Original languageEnglish
Pages (from-to)126-134
Number of pages9
JournalPlant Physiology
Volume133
Issue number1
DOIs
Publication statusPublished - Sep 2003
Externally publishedYes

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