Diffusion-Limited Growth of Microbial Colonies

Hayden Tronnolone, Alexander Tam, Zoltán Szenczi, J. E.F. Green, Sanjeeva Balasuriya, Ee Lin Tek, Jennifer M. Gardner, Joanna F. Sundstrom, Vladimir Jiranek, Stephen G. Oliver, Benjamin J. Binder

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Abstract

The emergence of diffusion-limited growth (DLG) within a microbial colony on a solid substrate is studied using a combination of mathematical modelling and experiments. Using an agent-based model of the interaction between microbial cells and a diffusing nutrient, it is shown that growth directed towards a nutrient source may be used as an indicator that DLG is influencing the colony morphology. A continuous reaction-diffusion model for microbial growth is employed to identify the parameter regime in which DLG is expected to arise. Comparisons between the model and experimental data are used to argue that the bacterium Bacillus subtilis can undergo DLG, while the yeast Saccharomyces cerevisiae cannot, and thus the non-uniform growth exhibited by this yeast must be caused by the pseudohyphal growth mode rather than limited nutrient availability. Experiments testing directly for DLG features in yeast colonies are used to confirm this hypothesis.

Original languageEnglish
Article number5992
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 1 Dec 2018
Externally publishedYes

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  • Cite this

    Tronnolone, H., Tam, A., Szenczi, Z., Green, J. E. F., Balasuriya, S., Tek, E. L., Gardner, J. M., Sundstrom, J. F., Jiranek, V., Oliver, S. G., & Binder, B. J. (2018). Diffusion-Limited Growth of Microbial Colonies. Scientific Reports, 8, [5992]. https://doi.org/10.1038/s41598-018-23649-z