Digital PCR: A brief history

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    Digital PCR for quantification of a target of interest has been independently developed several times, being described in 1990 and 1991 using the term "limiting dilution PCR" and in 1999 using the term "digital PCR". It came into use in the decade following its first development but its use was cut short by the description of real-time PCR in 1996. However digital PCR has now had a renaissance due to the recent development of new instruments and chemistry which have made it a much simpler and more practical technique.

    Original languageEnglish
    Pages (from-to)1-2
    Number of pages2
    JournalBiomolecular Detection and Quantification
    Issue number1
    Publication statusPublished - Sept 2014

    Bibliographical note

    Open access under CC BY-NC-ND license.


    • Digital PCR
    • Limiting dilution
    • PCR


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