TY - JOUR
T1 - Direct identification of forensic body fluids using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
AU - Kamanna, Sathisha
AU - Henry, Julianne
AU - Voelcker, Nicholas
AU - Linacre, Adrian
AU - Kirkbride, Kenneth
PY - 2016/3/15
Y1 - 2016/3/15
N2 - The examination of body fluids such as blood, seminal fluid, urine or saliva is a key aspect of forensic science in regards to investigation of crimes against the person such as murder and rape. This article describes a streamlined and simplified approach for the identification of body fluids using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) that avoids fractionation or isolation of proteins. It is applicable to the examination of microliter quantities (or less) of neat fluids, extracts or suspensions of dried fluids or deposits of fluids in situ on tufts of fibers plucked from fabrics, which has practical application in the examination of crime-related items such as underwear or face masks. Hemoglobin was used as a marker for blood, α-amylase for saliva, semenogelins, prostate-specific antigen and acid phosphatase for seminal fluid and uromodulin for urine. Aged stains were successfully analyzed; blood proteins were easily detected in an 11-year-old blood stain.
AB - The examination of body fluids such as blood, seminal fluid, urine or saliva is a key aspect of forensic science in regards to investigation of crimes against the person such as murder and rape. This article describes a streamlined and simplified approach for the identification of body fluids using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) that avoids fractionation or isolation of proteins. It is applicable to the examination of microliter quantities (or less) of neat fluids, extracts or suspensions of dried fluids or deposits of fluids in situ on tufts of fibers plucked from fabrics, which has practical application in the examination of crime-related items such as underwear or face masks. Hemoglobin was used as a marker for blood, α-amylase for saliva, semenogelins, prostate-specific antigen and acid phosphatase for seminal fluid and uromodulin for urine. Aged stains were successfully analyzed; blood proteins were easily detected in an 11-year-old blood stain.
KW - Body fluids
KW - Fabric substrates
KW - Forensic science
KW - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS)
KW - Protein biomarkers
UR - http://www.scopus.com/inward/record.url?scp=84960105439&partnerID=8YFLogxK
U2 - 10.1016/j.ijms.2016.01.002
DO - 10.1016/j.ijms.2016.01.002
M3 - Article
SN - 1387-3806
VL - 397-398
SP - 18
EP - 26
JO - International Journal of Mass Spectrometry
JF - International Journal of Mass Spectrometry
ER -