Direct PCR Improves the Recovery of DNA from Various Substrates

Jennifer Templeton, Duncan Taylor, Oliva Handt, Pawel Skuza, Adrian Linacre

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    This study reports on the comparison of a standard extraction process with the direct PCR approach of processing low-level DNA swabs typical in forensic investigations. Varying concentrations of control DNA were deposited onto three commonly encountered substrates, brass, plastic, and glass, left to dry, and swabbed using premoistened DNA-free nylon FLOQswabs. Swabs (n = 90) were either processed using the DNA IQ kit or, for direct PCR, swab fibers (~2 mm2) were added directly to the PCR with no prior extraction. A significant increase in the height of the alleles (p < 0.005) was observed when using the direct PCR approach over the extraction methodology when controlling for surface type and mass of DNA deposited. The findings indicate the potential use of direct PCR for increasing the PCR product obtained from low-template DNA samples in addition to minimizing contamination and saving resources.

    Original languageEnglish
    Pages (from-to)1558-1562
    Number of pages5
    JournalJournal of Forensic Sciences
    Volume60
    Issue number6
    DOIs
    Publication statusPublished - 1 Nov 2015

    Keywords

    • Direct Polymerase Chain Reaction
    • DNA typing
    • Forensic science
    • Human identification
    • Short tandem repeats
    • Trace DNA

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