Disruption and functional analysis of six ORFs on chromosome X11 of Saccharomyces cerevisiae: YLR124w, YLR125w, YLR126c, YLR127c, YLR128w and YLR129w

Martin Willer; Matthieu Regnacq; Peter J Reid; John R Tyson; Cui Wei; Barrie M Wilkinson; Colin J Stirling

doi:10.1002/1097-0061(200011)16:15<1429::AID-YEA629>3.0.CO;2-S

Disruption and functional analysis of six ORFs on chromosome X11 of Saccharomyces cerevisiae: YLR124w, YLR125w, YLR126c, YLR127c, YLR128w and YLR129w

Martin Willer, Matthieu Regnacq, Peter J Reid, John R Tyson, Cui Wei, Barrie M Wilkinson, Colin J Stirling

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Abstract

In the context of the EUROFAN programme, we report the deletion and functional analysis of six open reading frames (ORFs) on the right arm of chromosome XII of Saccharomyces cerevisiae. Using a PCR-based gene replacement strategy, we have systematically deleted individual ORFs and subjected the heterozygous diploids and haploid knockout strains to basic genetic and phenotypic characterization. Two ORFs, YLR127c and YLR129w, are essential for viability, whereas no growth phenotype could be detected following deletion of YLR124w, YLR125w, YLR126c or YLR128w. For each of the individual ORFs, a kanMX4 replacement cassette and the corresponding cognate wild-type gene were cloned into appropriate plasmids.

Original language	English
Pages (from-to)	1429-1435
Number of pages	7
Journal	YEAST
Volume	16
Issue number	15
DOIs	https://doi.org/10.1002/1097-0061(200011)16:15<1429::AID-YEA629>3.0.CO;2-S
Publication status	Published - 2000

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10.1002/1097-0061(200011)16:15<1429::AID-YEA629>3.0.CO;2-SLicence: Unspecified

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title = "Disruption and functional analysis of six ORFs on chromosome X11 of Saccharomyces cerevisiae: YLR124w, YLR125w, YLR126c, YLR127c, YLR128w and YLR129w",

abstract = "In the context of the EUROFAN programme, we report the deletion and functional analysis of six open reading frames (ORFs) on the right arm of chromosome XII of Saccharomyces cerevisiae. Using a PCR-based gene replacement strategy, we have systematically deleted individual ORFs and subjected the heterozygous diploids and haploid knockout strains to basic genetic and phenotypic characterization. Two ORFs, YLR127c and YLR129w, are essential for viability, whereas no growth phenotype could be detected following deletion of YLR124w, YLR125w, YLR126c or YLR128w. For each of the individual ORFs, a kanMX4 replacement cassette and the corresponding cognate wild-type gene were cloned into appropriate plasmids.",

author = "Martin Willer and Matthieu Regnacq and Reid, {Peter J} and Tyson, {John R} and Cui Wei and Wilkinson, {Barrie M} and Stirling, {Colin J}",

year = "2000",

doi = "10.1002/1097-0061(200011)16:15<1429::AID-YEA629>3.0.CO;2-S",

language = "English",

volume = "16",

pages = "1429--1435",

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TY - JOUR

T1 - Disruption and functional analysis of six ORFs on chromosome X11 of Saccharomyces cerevisiae: YLR124w, YLR125w, YLR126c, YLR127c, YLR128w and YLR129w

AU - Willer, Martin

AU - Regnacq, Matthieu

AU - Reid, Peter J

AU - Tyson, John R

AU - Wei, Cui

AU - Wilkinson, Barrie M

AU - Stirling, Colin J

PY - 2000

Y1 - 2000

N2 - In the context of the EUROFAN programme, we report the deletion and functional analysis of six open reading frames (ORFs) on the right arm of chromosome XII of Saccharomyces cerevisiae. Using a PCR-based gene replacement strategy, we have systematically deleted individual ORFs and subjected the heterozygous diploids and haploid knockout strains to basic genetic and phenotypic characterization. Two ORFs, YLR127c and YLR129w, are essential for viability, whereas no growth phenotype could be detected following deletion of YLR124w, YLR125w, YLR126c or YLR128w. For each of the individual ORFs, a kanMX4 replacement cassette and the corresponding cognate wild-type gene were cloned into appropriate plasmids.

AB - In the context of the EUROFAN programme, we report the deletion and functional analysis of six open reading frames (ORFs) on the right arm of chromosome XII of Saccharomyces cerevisiae. Using a PCR-based gene replacement strategy, we have systematically deleted individual ORFs and subjected the heterozygous diploids and haploid knockout strains to basic genetic and phenotypic characterization. Two ORFs, YLR127c and YLR129w, are essential for viability, whereas no growth phenotype could be detected following deletion of YLR124w, YLR125w, YLR126c or YLR128w. For each of the individual ORFs, a kanMX4 replacement cassette and the corresponding cognate wild-type gene were cloned into appropriate plasmids.

U2 - 10.1002/1097-0061(200011)16:15<1429::AID-YEA629>3.0.CO;2-S

DO - 10.1002/1097-0061(200011)16:15<1429::AID-YEA629>3.0.CO;2-S

M3 - Article

SN - 0749-503X

VL - 16

SP - 1429

EP - 1435

JO - YEAST

JF - YEAST

IS - 15

ER -

Disruption and functional analysis of six ORFs on chromosome X11 of Saccharomyces cerevisiae: YLR124w, YLR125w, YLR126c, YLR127c, YLR128w and YLR129w

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