DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels

Dmitriy Khodakov, Leigh Thredgold, Claire Lenehan, Gunther Andersson, Hilton Kobus, Amanda Ellis

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional "tag sequence" linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes.

    Original languageEnglish
    Article number026503
    Pages (from-to)026503-1-026503-11
    Number of pages1
    JournalBiomicrofluidics
    Volume6
    Issue number2
    DOIs
    Publication statusPublished - 26 Apr 2012

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