DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels

Dmitriy Khodakov; Leigh Thredgold; Claire Lenehan; Gunther Andersson; Hilton Kobus; Amanda Ellis

doi:10.1063/1.4729131

DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels

Dmitriy Khodakov, Leigh Thredgold, Claire Lenehan, Gunther Andersson, Hilton Kobus, Amanda Ellis

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional "tag sequence" linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes.

Original language	English
Article number	026503
Pages (from-to)	026503-1-026503-11
Number of pages	1
Journal	Biomicrofluidics
Volume	6
Issue number	2
DOIs	https://doi.org/10.1063/1.4729131
Publication status	Published - 26 Apr 2012

Access to Document

10.1063/1.4729131

Cite this

@article{0822298021744db18e2177ff8ba41177,

title = "DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels",

abstract = "Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional {"}tag sequence{"} linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes.",

author = "Dmitriy Khodakov and Leigh Thredgold and Claire Lenehan and Gunther Andersson and Hilton Kobus and Amanda Ellis",

year = "2012",

month = apr,

day = "26",

doi = "10.1063/1.4729131",

language = "English",

volume = "6",

pages = "026503--1--026503--11",

journal = "Biomicrofluidics",

issn = "1932-1058",

publisher = "American Institute of Physics",

number = "2",

}

TY - JOUR

T1 - DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels

AU - Khodakov, Dmitriy

AU - Thredgold, Leigh

AU - Lenehan, Claire

AU - Andersson, Gunther

AU - Kobus, Hilton

AU - Ellis, Amanda

PY - 2012/4/26

Y1 - 2012/4/26

N2 - Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional "tag sequence" linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes.

AB - Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional "tag sequence" linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes.

UR - http://www.scopus.com/inward/record.url?scp=84863228018&partnerID=8YFLogxK

U2 - 10.1063/1.4729131

DO - 10.1063/1.4729131

M3 - Article

SN - 1932-1058

VL - 6

SP - 026503-1-026503-11

JO - Biomicrofluidics

JF - Biomicrofluidics

IS - 2

M1 - 026503

ER -

DNA capture-probe based separation of double-stranded polymerase chain reaction amplification products in poly(dimethylsiloxane) microfluidic channels

Abstract

Access to Document

Other files and links

Fingerprint

Cite this