DNA: Hair Analysis

Adrian Linacre; Renée Ottens

doi:10.1016/B978-0-12-800034-2.00157-9

DNA: Hair Analysis

Adrian Linacre, Renée Ottens

College of Science and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Hairs are encountered commonly in a forensic investigation and can be a source of DNA. The amount of DNA within a single hair is often less than the optimal amount required for nuclear DNA markers. Most of the DNA is within a root sheath with trace amounts of mitochondrial DNA in the shaft. Nuclear DNA testing targets highly repetitive DNA regions called microsatellites. The quantity of starting template required for nuclear DNA profiling is 1. ng, which may be present in the root sheath but not within the hair shaft. Mitochondrial loci are targeted on hair shafts due to their greater copy number.

Original language	English
Title of host publication	Encyclopedia of Forensic and Legal Medicine
Editors	Jason Payne-James, Roger W. Byard
Publisher	Elsevier
Pages	337-342 (volume 2)
Number of pages	6
Edition	2nd
ISBN (Electronic)	9780128000557
ISBN (Print)	9780128000342
DOIs	https://doi.org/10.1016/B978-0-12-800034-2.00157-9
Publication status	Published - 2016

Keywords

Allele
Anagen
Animal testing
Catagen
Cortex
Direct PCR
Forensic science
Hair
SA
Medulla
Mitochondrial DNA
PCR
STR typing
Telogen
Hypervariable region

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title = "DNA: Hair Analysis",

abstract = "Hairs are encountered commonly in a forensic investigation and can be a source of DNA. The amount of DNA within a single hair is often less than the optimal amount required for nuclear DNA markers. Most of the DNA is within a root sheath with trace amounts of mitochondrial DNA in the shaft. Nuclear DNA testing targets highly repetitive DNA regions called microsatellites. The quantity of starting template required for nuclear DNA profiling is 1. ng, which may be present in the root sheath but not within the hair shaft. Mitochondrial loci are targeted on hair shafts due to their greater copy number.",

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KW - Anagen

KW - Animal testing

KW - Catagen

KW - Cortex

KW - Direct PCR

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KW - SA

KW - Medulla

KW - Mitochondrial DNA

KW - PCR

KW - STR typing

KW - Telogen

KW - Hypervariable region

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BT - Encyclopedia of Forensic and Legal Medicine

A2 - Payne-James, Jason

A2 - Byard, Roger W.

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ER -

DNA: Hair Analysis

Abstract

Keywords

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