DNA profiles from fingermarks

Jennifer Templeton, Adrian Linacre

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Criminal investigations would be considerably improved if DNA profiles could be routinely generated from single fingermarks. Here we report a direct DNA profiling method that was able to generate interpretable profiles from 71% of 170 fingermarks. The data are based on fingermarks from all 5 digits of 34 individuals. DNA was obtained from the fingermarks using a swab moistened with Triton-X, and the fibers were added directly to one of two commercial DNA profiling kits. All profiles were obtained without increasing the number of amplification cycles; therefore, our method is ideally suited for adoption by the forensic science community. We indicate the use of the technique in a criminal case in which a DNA profile was generated from a fingermark on tape that was wrapped around a drug seizure. Our direct DNA profiling approach is rapid and able to generate profiles from touched items when current forensic practices have little chance of success.

    Original languageEnglish
    Pages (from-to)259-266
    Number of pages8
    JournalBiotechniques
    Volume57
    Issue number5
    DOIs
    Publication statusPublished - 1 Nov 2014

    Keywords

    • direct PCR
    • DNA profiling
    • fingermarks
    • NGM SElect
    • Profiler Plus
    • short tandem repeats

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