DNA profiles from matchsticks

Belinda Martin; Renee Blackie; Adrian Linacre

doi:10.1080/00450618.2019.1569138

DNA profiles from matchsticks

Belinda Martin, Renee Blackie, Adrian Linacre

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Matchsticks may be found in forensic investigations, and here we report on a method to generate informative DNA profiles from a matchstick. Matchsticks were struck or held emulating striking, cellular material was removed using a tape-lift method and DNA profiles were generated from a small portion of the tape-lift using either direct PCR amplification or post-extraction PCR. Performing an extraction from the tape-lift prior to PCR resulted in 62% of profiles being informative, compared with 86% held and 97% struck matchsticks that underwent direct PCR. Incorporation of a tape-lift method into matchstick analysis allows effective collection of cellular material for the generation of genetic information from the striker of the matchstick.

Original language	English
Pages (from-to)	S18-S22
Number of pages	5
Journal	Australian Journal of Forensic Sciences
Volume	51
Issue number	sup1
DOIs	https://doi.org/10.1080/00450618.2019.1569138
Publication status	Published - 22 Jan 2019

Keywords

direct PCR
Forensic science
matchstick
STR profiling

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10.1080/00450618.2019.1569138Licence: In Copyright

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title = "DNA profiles from matchsticks",

abstract = "Matchsticks may be found in forensic investigations, and here we report on a method to generate informative DNA profiles from a matchstick. Matchsticks were struck or held emulating striking, cellular material was removed using a tape-lift method and DNA profiles were generated from a small portion of the tape-lift using either direct PCR amplification or post-extraction PCR. Performing an extraction from the tape-lift prior to PCR resulted in 62% of profiles being informative, compared with 86% held and 97% struck matchsticks that underwent direct PCR. Incorporation of a tape-lift method into matchstick analysis allows effective collection of cellular material for the generation of genetic information from the striker of the matchstick.",

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AU - Linacre, Adrian

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N2 - Matchsticks may be found in forensic investigations, and here we report on a method to generate informative DNA profiles from a matchstick. Matchsticks were struck or held emulating striking, cellular material was removed using a tape-lift method and DNA profiles were generated from a small portion of the tape-lift using either direct PCR amplification or post-extraction PCR. Performing an extraction from the tape-lift prior to PCR resulted in 62% of profiles being informative, compared with 86% held and 97% struck matchsticks that underwent direct PCR. Incorporation of a tape-lift method into matchstick analysis allows effective collection of cellular material for the generation of genetic information from the striker of the matchstick.

AB - Matchsticks may be found in forensic investigations, and here we report on a method to generate informative DNA profiles from a matchstick. Matchsticks were struck or held emulating striking, cellular material was removed using a tape-lift method and DNA profiles were generated from a small portion of the tape-lift using either direct PCR amplification or post-extraction PCR. Performing an extraction from the tape-lift prior to PCR resulted in 62% of profiles being informative, compared with 86% held and 97% struck matchsticks that underwent direct PCR. Incorporation of a tape-lift method into matchstick analysis allows effective collection of cellular material for the generation of genetic information from the striker of the matchstick.

KW - direct PCR

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KW - STR profiling

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JO - Australian Journal of Forensic Sciences

JF - Australian Journal of Forensic Sciences

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DNA profiles from matchsticks

Abstract

Keywords

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