DNA profiles from matchsticks

Belinda Martin, Renee Blackie, Adrian Linacre

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Matchsticks may be found in forensic investigations, and here we report on a method to generate informative DNA profiles from a matchstick. Matchsticks were struck or held emulating striking, cellular material was removed using a tape-lift method and DNA profiles were generated from a small portion of the tape-lift using either direct PCR amplification or post-extraction PCR. Performing an extraction from the tape-lift prior to PCR resulted in 62% of profiles being informative, compared with 86% held and 97% struck matchsticks that underwent direct PCR. Incorporation of a tape-lift method into matchstick analysis allows effective collection of cellular material for the generation of genetic information from the striker of the matchstick.

Original languageEnglish
Pages (from-to)S18-S22
Number of pages5
JournalAustralian Journal of Forensic Sciences
Volume51
Issue numbersup1
DOIs
Publication statusPublished - 22 Jan 2019

Keywords

  • direct PCR
  • Forensic science
  • matchstick
  • STR profiling

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