DNA typing in wildlife crime: recent developments in species identification

Shanan Tobe, Adrian Linacre

    Research output: Contribution to journalReview articlepeer-review

    31 Citations (Scopus)

    Abstract

    Species identification has become a tool in the investigation of acts of alleged wildlife crimes. This review details the steps required in DNA testing in wildlife crime investigations and highlights recent developments where not only can individual species be identified within a mixture of species but multiple species can be identified simultaneously. 'What species is this?' is a question asked frequently in wildlife crime investigations. Depending on the material being examined, DNA analysis may offer the best opportunity to answer this question. Species testing requires the comparison of the DNA type from the unknown sample to DNA types on a database. The areas of DNA tested are on the mitochondria and include predominantly the cytochrome b gene and the cytochrome oxidase I gene. Standard analysis requires the sequencing of part of one of these genes and comparing the sequence to that held on a repository of DNA sequences such as the GenBank database. Much of the DNA sequence of either of these two genes is conserved with only parts being variable. A recent development is to target areas of those sequences that are specific to a species; this can increase the sensitivity of the test with no loss of specificity. The benefit of targeting species specific sequences is that within a mixture of two of more species, the individual species within the mixture can be identified. This identification would not be possible using standard sequencing. These new developments can lead to a greater number of samples being tested in alleged wildlife crimes.

    Original languageEnglish
    Pages (from-to)195-206
    Number of pages12
    JournalForensic Science, Medicine, and Pathology
    Volume6
    Issue number3
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Cytochrome b
    • Cytochrome oxidase I
    • DNA mixtures
    • Non-human DNA
    • Species identification

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