Drug and chemical glucosidation by control supersomes and membranes from spodoptera frugiperda (sf) 9 cells: Implications for the apparent glucuronidation of xenobiotics by UDP-glucuronosyltransferase 1A5

Nuy Chau, Leyla Kaya, Benjamin C. Lewis, Peter I. Mackenzie, John O. Miners

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Accumulating evidence indicates that several human UDPglucuronosyltransferase (UGT) enzymes catalyze both glucuronidation and glucosidation reactions. Baculovirus-infected insect cells [Trichoplusia ni and Spodoptera frugiperda (Sf9)] are used widely for the expression of recombinant human UGT enzymes. Following the observation that control Supersomes (c-SUP) express a native enzyme capable of glucosidating morphine, we characterized the glucosidation of a series of aglycones with a hydroxyl (aliphatic or phenolic), carboxylic acid, or amine functional group by c-SUP and membranes from uninfected Sf9 cells. Although both enzyme sources glucosidated the phenolic substrates investigated, albeit with differing activities, differences were observed in the selectivities of the native UDP-glucosyltransferases toward aliphatic alcohols, carboxylic acids, and amines. For example, zidovudine was solely glucosidated by c-SUP. By contrast, c-SUP lacked activity toward the amines lamotrigine and trifluoperazine and did not form the acyl glucoside of mycophenolic acid, reactions all catalyzed by uninfected Sf9 membranes. Glucosidation intrinsic clearances were high for several substrates, notably 1-hydroxypyrene (∼1400-1900 ml/min×mg). The results underscore the importance of including control cell membranes in the investigation of drug and chemical glucosidation by UGT enzymes expressed in T. ni (High-Five) and Sf9 cells. In a coincident study, we observed that UGT1A5 expressed in Sf9, human embryonic kidney 293T, and COS7 cells lacked glucuronidation activity toward prototypic phenolic substrates. However, Sf9 cells expressing UGT1A5 glucosidated 1-hydroxypyrene with UDP-glucuronic acid as the cofactor, presumably due to the presence of UDP-glucose as an impurity. Artifactual glucosidation may explain, at least in part, a previous report of phenolic glucuronidation by UGT1A5.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalDrug Metabolism and Disposition
Volume47
Issue number3
DOIs
Publication statusPublished - 1 Mar 2019

Keywords

  • enzyme
  • glucuronidation
  • glucuronic acid
  • glucosidation

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