TY - JOUR
T1 - Effect of alendronate-loaded bone cement on osteoblast
AU - Song, Deye
AU - Ni, Jiangdong
AU - Mao, Xinzhan
AU - Ding, Muliang
PY - 2014/3
Y1 - 2014/3
N2 - Objective: To determine the effect of bone cement, with different amounts of alendronate on osteoblast, and determine the cytotoxicity of alendronate-integrated bone cement from the viewpoint of cell biology. Methods: According to different additions (0, 10, 50, 100, 500, 1000 mg) of alendronate in 50 g Cemex®XL bone cement powder, the experiments were divided into 6 groups, namely G0-G5 groups. In all groups, the adhesive capacity of osteoblast-like cells MG-63 was evaluated by electron microscope, the optical density (OD) value of cells by MTT colorimetry method, the alkaline phosphatase activity (AKP) by AKP assay kit, the apoptosis rates by Annexin-V-FITC apoptosis detection kit, and the bone mineralization potentiality by phase contrast microscope. Results: The adhesive capacity of MG-63 was good in all groups. Compared with the G0 group, the cell apoptosis was inhibited in G1-G4 groups while in G5 group the cell apoptosis was promoted and cell proliferation was inhibited (P<0.05). In all groups, no significant difference was found in alkaline phosphatase activity and bone mineralization potentiality (P>0.05). Conclusion: Less than 500 mg alendronate added in Cemex®XL 50 g bone cement powder has no cytotoxicity on osteoblasts.
AB - Objective: To determine the effect of bone cement, with different amounts of alendronate on osteoblast, and determine the cytotoxicity of alendronate-integrated bone cement from the viewpoint of cell biology. Methods: According to different additions (0, 10, 50, 100, 500, 1000 mg) of alendronate in 50 g Cemex®XL bone cement powder, the experiments were divided into 6 groups, namely G0-G5 groups. In all groups, the adhesive capacity of osteoblast-like cells MG-63 was evaluated by electron microscope, the optical density (OD) value of cells by MTT colorimetry method, the alkaline phosphatase activity (AKP) by AKP assay kit, the apoptosis rates by Annexin-V-FITC apoptosis detection kit, and the bone mineralization potentiality by phase contrast microscope. Results: The adhesive capacity of MG-63 was good in all groups. Compared with the G0 group, the cell apoptosis was inhibited in G1-G4 groups while in G5 group the cell apoptosis was promoted and cell proliferation was inhibited (P<0.05). In all groups, no significant difference was found in alkaline phosphatase activity and bone mineralization potentiality (P>0.05). Conclusion: Less than 500 mg alendronate added in Cemex®XL 50 g bone cement powder has no cytotoxicity on osteoblasts.
KW - Alendronate
KW - Amount
KW - Bisphosphonate
KW - Bone cement
KW - Osteoblast
UR - http://www.scopus.com/inward/record.url?scp=84899482790&partnerID=8YFLogxK
U2 - 10.11817/j.issn.1672-7347.2014.03.011
DO - 10.11817/j.issn.1672-7347.2014.03.011
M3 - Article
SN - 2095-2899
VL - 39
SP - 290
EP - 295
JO - Journal of Central South University
JF - Journal of Central South University
IS - 3
ER -