Effect of cytosine arabinoside triphosphate on deoxyribonucleic acid synthesis in permealysed cells from ehrlich ascites tumour. Studies of phosphorylated drug metabolites on quasi-normal deoxyribonucleic acid replication

Leigh A. Burgoyne

doi:10.1016/0006-2952(74)90374-8

Effect of cytosine arabinoside triphosphate on deoxyribonucleic acid synthesis in permealysed cells from ehrlich ascites tumour. Studies of phosphorylated drug metabolites on quasi-normal deoxyribonucleic acid replication

Leigh A. Burgoyne

College of Science and Engineering

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Cytosine arabinoside triphosphate (ara-CTP) is a strong inhibitor of DNA synthesis in permealysed Ehrlich ascites cells, and this effect is rapidly reversed by dCTP but not by d-cytidine. Cytosine arabinoside is a weak inhibitor of DNA synthesis in permealysed Ehrlich ascites cells, and this effect is reversed by both dCTP and d-cytidine. A slightly improved assay system is described, and the permealysed ascites cells are shown to slowly lose activity during assays although DNA polymerase activity is not lost. An explanation of the effect of ara-CTP on DNA synthesis in permealysed cells and other systems is presented.

Original language	English
Pages (from-to)	1619-1627
Number of pages	9
Journal	Biochemical Pharmacology
Volume	23
Issue number	11
DOIs	https://doi.org/10.1016/0006-2952(74)90374-8
Publication status	Published - 1 Jun 1974

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title = "Effect of cytosine arabinoside triphosphate on deoxyribonucleic acid synthesis in permealysed cells from ehrlich ascites tumour. Studies of phosphorylated drug metabolites on quasi-normal deoxyribonucleic acid replication",

abstract = "Cytosine arabinoside triphosphate (ara-CTP) is a strong inhibitor of DNA synthesis in permealysed Ehrlich ascites cells, and this effect is rapidly reversed by dCTP but not by d-cytidine. Cytosine arabinoside is a weak inhibitor of DNA synthesis in permealysed Ehrlich ascites cells, and this effect is reversed by both dCTP and d-cytidine. A slightly improved assay system is described, and the permealysed ascites cells are shown to slowly lose activity during assays although DNA polymerase activity is not lost. An explanation of the effect of ara-CTP on DNA synthesis in permealysed cells and other systems is presented.",

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Effect of cytosine arabinoside triphosphate on deoxyribonucleic acid synthesis in permealysed cells from ehrlich ascites tumour. Studies of phosphorylated drug metabolites on quasi-normal deoxyribonucleic acid replication. / Burgoyne, Leigh A.
In: Biochemical Pharmacology, Vol. 23, No. 11, 01.06.1974, p. 1619-1627.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effect of cytosine arabinoside triphosphate on deoxyribonucleic acid synthesis in permealysed cells from ehrlich ascites tumour. Studies of phosphorylated drug metabolites on quasi-normal deoxyribonucleic acid replication

AU - Burgoyne, Leigh A.

PY - 1974/6/1

Y1 - 1974/6/1

N2 - Cytosine arabinoside triphosphate (ara-CTP) is a strong inhibitor of DNA synthesis in permealysed Ehrlich ascites cells, and this effect is rapidly reversed by dCTP but not by d-cytidine. Cytosine arabinoside is a weak inhibitor of DNA synthesis in permealysed Ehrlich ascites cells, and this effect is reversed by both dCTP and d-cytidine. A slightly improved assay system is described, and the permealysed ascites cells are shown to slowly lose activity during assays although DNA polymerase activity is not lost. An explanation of the effect of ara-CTP on DNA synthesis in permealysed cells and other systems is presented.

AB - Cytosine arabinoside triphosphate (ara-CTP) is a strong inhibitor of DNA synthesis in permealysed Ehrlich ascites cells, and this effect is rapidly reversed by dCTP but not by d-cytidine. Cytosine arabinoside is a weak inhibitor of DNA synthesis in permealysed Ehrlich ascites cells, and this effect is reversed by both dCTP and d-cytidine. A slightly improved assay system is described, and the permealysed ascites cells are shown to slowly lose activity during assays although DNA polymerase activity is not lost. An explanation of the effect of ara-CTP on DNA synthesis in permealysed cells and other systems is presented.

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VL - 23

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JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 11

ER -

Effect of cytosine arabinoside triphosphate on deoxyribonucleic acid synthesis in permealysed cells from ehrlich ascites tumour. Studies of phosphorylated drug metabolites on quasi-normal deoxyribonucleic acid replication

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