TY - JOUR
T1 - Effect of dietary copper on the growth performance, non-specific immunity and resistance to Aeromonas hydrophila of juvenile Chinese mitten crab, Eriocheir sinensis
AU - Sun, Shengming
AU - Qin, Jianguang
AU - Chen, Liqiao
AU - Ge, XP
AU - Jiang, Haibo
PY - 2013/5
Y1 - 2013/5
N2 - An 8-week feeding trial was conducted to determine the dietary copper (Cu) on growth performance and immune responses of juvenile Chinese mitten crab Eriocheir sinensis. Six semi-purified diets with six copper levels (1.88, 11.85, 20.78, 40.34, 79.56 and 381.2 mg kg-1 diet) of CuSO4·5H2O were fed to E. sinensis (0.45 ± 0.01 g). Each diet was fed to the crab in five replicates. The crab fed diets with 20.78 and 40.34 mg Cu kg-1 diet had significantly greater weight gain and hemolymph oxyhemocyanin content than those fed diets with 1.88 and 381.2 mg Cu kg-1 diet. Survival rates of crab were not significantly different between all treatment groups. The activities of copper-zinc superoxide dismutase (Cu-Zn SOD), phenoloxidase (PO), and total hemocyte count (THC) significantly increased when the supplementation of dietary copper reached 20.78-40.34 mg Cu kg-1 diets. In the bacteria challenge experiment with Aeromonas hydrophila, survival rates significantly increased and reached a plateau when the dietary copper increased from 1.88 to 40.34 mg kg-1, whereas significantly decreased when the dietary copper increased from 40.34 to 381.2 mg kg-1. This study indicates that the level of dietary copper is important in regulating growth and immune response in crab.
AB - An 8-week feeding trial was conducted to determine the dietary copper (Cu) on growth performance and immune responses of juvenile Chinese mitten crab Eriocheir sinensis. Six semi-purified diets with six copper levels (1.88, 11.85, 20.78, 40.34, 79.56 and 381.2 mg kg-1 diet) of CuSO4·5H2O were fed to E. sinensis (0.45 ± 0.01 g). Each diet was fed to the crab in five replicates. The crab fed diets with 20.78 and 40.34 mg Cu kg-1 diet had significantly greater weight gain and hemolymph oxyhemocyanin content than those fed diets with 1.88 and 381.2 mg Cu kg-1 diet. Survival rates of crab were not significantly different between all treatment groups. The activities of copper-zinc superoxide dismutase (Cu-Zn SOD), phenoloxidase (PO), and total hemocyte count (THC) significantly increased when the supplementation of dietary copper reached 20.78-40.34 mg Cu kg-1 diets. In the bacteria challenge experiment with Aeromonas hydrophila, survival rates significantly increased and reached a plateau when the dietary copper increased from 1.88 to 40.34 mg kg-1, whereas significantly decreased when the dietary copper increased from 40.34 to 381.2 mg kg-1. This study indicates that the level of dietary copper is important in regulating growth and immune response in crab.
KW - Copper
KW - Eriocheir sinensis
KW - Growth
KW - Non-specific immunity
KW - Oxyhemocyanin
UR - http://www.scopus.com/inward/record.url?scp=84875808866&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2013.01.021
DO - 10.1016/j.fsi.2013.01.021
M3 - Article
VL - 34
SP - 1195
EP - 1201
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
SN - 1050-4648
IS - 5
ER -