Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherixhia coli K-12

Renato Morona, Melissa H. Brown, Jane Yeadon, Michael W. Heuzenroeder, Paul A. Manning

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae O-antigen is assembled onto the E. coli LPS and that at least two glucoses are needed in the core in order to achieve a high level of production. These data are consistent with the reported presence of glucose in the V. cholerae LPS-core-oligosaccharide region.

Original languageEnglish
Pages (from-to)279-285
Number of pages7
JournalFEMS Microbiology Letters
Volume82
Issue number3
DOIs
Publication statusPublished - 1 Aug 1991
Externally publishedYes

Keywords

  • Glucose
  • rfa Gene
  • rfb Gene
  • Silver stain
  • Transformation
  • Vibrio cholerae
  • Western blot

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