Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherixhia coli K-12

Renato Morona; Melissa H. Brown; Jane Yeadon; Michael W. Heuzenroeder; Paul A. Manning

doi:10.1111/j.1574-6968.1991.tb04895.x

Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherixhia coli K-12

Renato Morona
, Melissa H. Brown
, Jane Yeadon
, Michael W. Heuzenroeder
, Paul A. Manning

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae O-antigen is assembled onto the E. coli LPS and that at least two glucoses are needed in the core in order to achieve a high level of production. These data are consistent with the reported presence of glucose in the V. cholerae LPS-core-oligosaccharide region.

Original language	English
Pages (from-to)	279-285
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	82
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04895.x
Publication status	Published - 1 Aug 1991
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Glucose
rfa Gene
rfb Gene
Silver stain
Transformation
Vibrio cholerae
Western blot

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title = "Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherixhia coli K-12",

abstract = "The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae O-antigen is assembled onto the E. coli LPS and that at least two glucoses are needed in the core in order to achieve a high level of production. These data are consistent with the reported presence of glucose in the V. cholerae LPS-core-oligosaccharide region.",

keywords = "Glucose, rfa Gene, rfb Gene, Silver stain, Transformation, Vibrio cholerae, Western blot",

author = "Renato Morona and Brown, \{Melissa H.\} and Jane Yeadon and Heuzenroeder, \{Michael W.\} and Manning, \{Paul A.\}",

year = "1991",

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doi = "10.1111/j.1574-6968.1991.tb04895.x",

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T1 - Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherixhia coli K-12

AU - Morona, Renato

AU - Brown, Melissa H.

AU - Yeadon, Jane

AU - Heuzenroeder, Michael W.

AU - Manning, Paul A.

PY - 1991/8/1

Y1 - 1991/8/1

N2 - The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae O-antigen is assembled onto the E. coli LPS and that at least two glucoses are needed in the core in order to achieve a high level of production. These data are consistent with the reported presence of glucose in the V. cholerae LPS-core-oligosaccharide region.

AB - The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae O-antigen is assembled onto the E. coli LPS and that at least two glucoses are needed in the core in order to achieve a high level of production. These data are consistent with the reported presence of glucose in the V. cholerae LPS-core-oligosaccharide region.

KW - Glucose

KW - rfa Gene

KW - rfb Gene

KW - Silver stain

KW - Transformation

KW - Vibrio cholerae

KW - Western blot

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M3 - Article

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AN - SCOPUS:0026425385

SN - 0378-1097

VL - 82

SP - 279

EP - 285

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 3

ER -

Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherixhia coli K-12

Abstract

UN SDGs

Keywords

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