TY - JOUR
T1 - Effect of sample storage temperature and buffer formulation on faecal immunochemical test haemoglobin measurements.
AU - Symonds, Erin
AU - Cole, Stephen
AU - Bastin, Dawn
AU - Fraser, Robert
AU - Young, Graeme
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Objectives: Faecal immunochemical test accuracy may be adversely affected when samples are exposed to high temperatures. This study evaluated the effect of two sample collection buffer formulations (OC-Sensor, Eiken) and storage temperatures on faecal haemoglobin readings. Methods: Faecal immunochemical test samples returned in a screening programme and with 510 mg Hb/g faeces in either the original or new formulation haemoglobin stabilizing buffer were stored in the freezer, refrigerator, or at room temperature (22C–24C), and reanalysed after 1–14 days. Samples in the new buffer were also reanalysed after storage at 35C and 50C. Results were expressed as percentage of the initial concentration, and the number of days that levels were maintained to at least 80% was calculated. Results: Haemoglobin concentrations were maintained above 80% of their initial concentration with both freezer and refrigerator storage, regardless of buffer formulation or storage duration. Stability at room temperature was significantly better in the new buffer, with haemoglobin remaining above 80% for 20 days compared with six days in the original buffer. Storage at 35Cor 50C in the new buffer maintained haemoglobin above 80% for eight and two days, respectively. Conclusion: The new formulation buffer has enhanced haemoglobin stabilizing properties when samples are exposed to temperatures greater than 22C.
AB - Objectives: Faecal immunochemical test accuracy may be adversely affected when samples are exposed to high temperatures. This study evaluated the effect of two sample collection buffer formulations (OC-Sensor, Eiken) and storage temperatures on faecal haemoglobin readings. Methods: Faecal immunochemical test samples returned in a screening programme and with 510 mg Hb/g faeces in either the original or new formulation haemoglobin stabilizing buffer were stored in the freezer, refrigerator, or at room temperature (22C–24C), and reanalysed after 1–14 days. Samples in the new buffer were also reanalysed after storage at 35C and 50C. Results were expressed as percentage of the initial concentration, and the number of days that levels were maintained to at least 80% was calculated. Results: Haemoglobin concentrations were maintained above 80% of their initial concentration with both freezer and refrigerator storage, regardless of buffer formulation or storage duration. Stability at room temperature was significantly better in the new buffer, with haemoglobin remaining above 80% for 20 days compared with six days in the original buffer. Storage at 35Cor 50C in the new buffer maintained haemoglobin above 80% for eight and two days, respectively. Conclusion: The new formulation buffer has enhanced haemoglobin stabilizing properties when samples are exposed to temperatures greater than 22C.
KW - colorectal cancer screening
KW - faecal immunochemical test
KW - haemoglobin stability
KW - Occult blood
KW - temperature
UR - http://www.scopus.com/inward/record.url?scp=85046461321&partnerID=8YFLogxK
U2 - 10.1177/0969141316686808
DO - 10.1177/0969141316686808
M3 - Article
SN - 0969-1413
VL - 24
SP - 176
EP - 181
JO - Journal of Medical Screening
JF - Journal of Medical Screening
IS - 4
ER -