Effect of short-and long-term phosphate stress on the non-phosphorylating pathway of mitochondrial electron transport in Arabidopsis thaliana

Vivek Vijayraghavan, Kathleen Soole

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    Wild-type Arabidopsis thaliana L. seedlings generated in liquid culture were subjected to long-and short-term phosphate (Pi) stress to assess the response of the non-phosphorylating pathway of mitochondrial electron transport, comprising the internal (NDin) and external (NDex) non-phosphorylating NAD(P)H dehydrogenases and the alternative oxidase (AOX). Tissue Pi levels decreased but anthocyanin levels increased in both short-and long-term Pi stress. Oxidative damage was either lower or unchanged in response to short-and long-term Pi term stress. NDin and AOX capacity were elevated only in response to long-term Pi stress whereas NDex capacity was elevated only in response to short-term Pi stress. The only ND genes with elevated transcript levels in response to both short-and long-term Pi stress were Atnda2 (an NDin) and Atndb2 (an NDex). The binding site for PHR1, a transcription factor involved in the Pi stress response, was found in the promoter regions of Atnda2 and Atndb2. Results of this study indicate that a non-phosphorylating mitochondrial electron transport chain consisting of NDA2, NDB2 and AOX is synthesised to maintain respiratory electron flow through the mitochondrial electron transport chain during Pi stress.

    Original languageEnglish
    Pages (from-to)455-466
    Number of pages12
    JournalFunctional Plant Biology
    Volume37
    Issue number5
    DOIs
    Publication statusPublished - 2010

    Keywords

    • alternative oxidase
    • non-phosphorylating NAD(P)H dehydrogenases
    • oxidative stress
    • phosphate deprivation
    • reactive oxygen species

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