Effect of ultrafine-grained titanium surfaces on adhesion of bacteria

Vi Khanh Truong; Stuart Rundell; Rimma Lapovok; Yuri Estrin; James Y. Wang; Christopher C. Berndt; David G. Barnes; Christopher J. Fluke; Russell J. Crawford; Elena P. Ivanova

doi:10.1007/s00253-009-1944-5

Effect of ultrafine-grained titanium surfaces on adhesion of bacteria

Vi Khanh Truong, Stuart Rundell, Rimma Lapovok, Yuri Estrin, James Y. Wang, Christopher C. Berndt, David G. Barnes, Christopher J. Fluke, Russell J. Crawford, Elena P. Ivanova

Research output: Contribution to journal › Article › peer-review

110 Citations (Scopus)

Abstract

The influence of the ultrafine crystallinity of commercial purity grade 2 (as-received) titanium and titanium modified by equal channel angular pressing (modified titanium) on bacterial attachment was studied. A topographic profile analysis of the surface of the modified titanium revealed a complex morphology of the surface. Its prominent micro- and nano-scale features were 100-200-nm-scale undulations with 10-15 μm spacing. The undulating surfaces were nano-smooth, with height variations not exceeding 5-10 nm. These surface topography characteristics were distinctly different from those of the as-received samples, where broad valleys (up to 40-60 μm) were detected, whose inner surfaces exhibited asperities approximately 100 nm in height spaced at 1-2 μm. It was found that each of the three bacteria strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 68.5, Pseudomonas aeruginosa ATCC 9025 and Escherichia coli K12, responded differently to the two types of titanium surfaces. Extreme grain refinement by ECAP resulted in substantially increased numbers of cells attached to the surface compared to as-received titanium. This enhanced degree of attachment was accompanied with an increased level of extracellular polymeric substances (EPS) production by the bacteria.

Original language	English
Pages (from-to)	925-937
Number of pages	13
Journal	Applied Microbiology and Biotechnology
Volume	83
Issue number	5
DOIs	https://doi.org/10.1007/s00253-009-1944-5
Publication status	Published - Jul 2009
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgment This study was supported in part by the Australian Research Council (ARC).

Keywords

Bacterial adhesion
Equal channel angular pressing (ECAP)
Escherichia coli
Pseudomonas aeruginosa
Staphylococcus aureus
Titanium surfaces

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title = "Effect of ultrafine-grained titanium surfaces on adhesion of bacteria",

abstract = "The influence of the ultrafine crystallinity of commercial purity grade 2 (as-received) titanium and titanium modified by equal channel angular pressing (modified titanium) on bacterial attachment was studied. A topographic profile analysis of the surface of the modified titanium revealed a complex morphology of the surface. Its prominent micro- and nano-scale features were 100-200-nm-scale undulations with 10-15 μm spacing. The undulating surfaces were nano-smooth, with height variations not exceeding 5-10 nm. These surface topography characteristics were distinctly different from those of the as-received samples, where broad valleys (up to 40-60 μm) were detected, whose inner surfaces exhibited asperities approximately 100 nm in height spaced at 1-2 μm. It was found that each of the three bacteria strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 68.5, Pseudomonas aeruginosa ATCC 9025 and Escherichia coli K12, responded differently to the two types of titanium surfaces. Extreme grain refinement by ECAP resulted in substantially increased numbers of cells attached to the surface compared to as-received titanium. This enhanced degree of attachment was accompanied with an increased level of extracellular polymeric substances (EPS) production by the bacteria.",

keywords = "Bacterial adhesion, Equal channel angular pressing (ECAP), Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Titanium surfaces",

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AU - Truong, Vi Khanh

AU - Rundell, Stuart

AU - Lapovok, Rimma

AU - Estrin, Yuri

AU - Wang, James Y.

AU - Berndt, Christopher C.

AU - Barnes, David G.

AU - Fluke, Christopher J.

AU - Crawford, Russell J.

AU - Ivanova, Elena P.

N1 - Funding Information: Acknowledgment This study was supported in part by the Australian Research Council (ARC).

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N2 - The influence of the ultrafine crystallinity of commercial purity grade 2 (as-received) titanium and titanium modified by equal channel angular pressing (modified titanium) on bacterial attachment was studied. A topographic profile analysis of the surface of the modified titanium revealed a complex morphology of the surface. Its prominent micro- and nano-scale features were 100-200-nm-scale undulations with 10-15 μm spacing. The undulating surfaces were nano-smooth, with height variations not exceeding 5-10 nm. These surface topography characteristics were distinctly different from those of the as-received samples, where broad valleys (up to 40-60 μm) were detected, whose inner surfaces exhibited asperities approximately 100 nm in height spaced at 1-2 μm. It was found that each of the three bacteria strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 68.5, Pseudomonas aeruginosa ATCC 9025 and Escherichia coli K12, responded differently to the two types of titanium surfaces. Extreme grain refinement by ECAP resulted in substantially increased numbers of cells attached to the surface compared to as-received titanium. This enhanced degree of attachment was accompanied with an increased level of extracellular polymeric substances (EPS) production by the bacteria.

AB - The influence of the ultrafine crystallinity of commercial purity grade 2 (as-received) titanium and titanium modified by equal channel angular pressing (modified titanium) on bacterial attachment was studied. A topographic profile analysis of the surface of the modified titanium revealed a complex morphology of the surface. Its prominent micro- and nano-scale features were 100-200-nm-scale undulations with 10-15 μm spacing. The undulating surfaces were nano-smooth, with height variations not exceeding 5-10 nm. These surface topography characteristics were distinctly different from those of the as-received samples, where broad valleys (up to 40-60 μm) were detected, whose inner surfaces exhibited asperities approximately 100 nm in height spaced at 1-2 μm. It was found that each of the three bacteria strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 68.5, Pseudomonas aeruginosa ATCC 9025 and Escherichia coli K12, responded differently to the two types of titanium surfaces. Extreme grain refinement by ECAP resulted in substantially increased numbers of cells attached to the surface compared to as-received titanium. This enhanced degree of attachment was accompanied with an increased level of extracellular polymeric substances (EPS) production by the bacteria.

KW - Bacterial adhesion

KW - Equal channel angular pressing (ECAP)

KW - Escherichia coli

KW - Pseudomonas aeruginosa

KW - Staphylococcus aureus

KW - Titanium surfaces

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Effect of ultrafine-grained titanium surfaces on adhesion of bacteria

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Keywords

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