Effects of Broodstock Age and Sperm Collection Time over a Natural Spawning Period on Sperm Cryopreservation in Farmed Greenlip Abalone, Haliotis laevigata

Yi Liu, Xiaoxu Li, Tong Xu, Nicholas Robinson, Jianguang Qin

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    This study investigated the effects of broodstock age (2- and 3-yr-old), and sperm collection time at the beginning, middle, and end of a natural spawning period, on the ability of sperm to tolerate cryopreservation in farmed greenlip abalone, Haliotis laevigata. The quality of sperm was assessed by motility, fertilization rate, plasma membrane integrity, mitochondrial membrane potential (MMP), and acrosome integrity (AI). The sperm collected at the middle of a natural spawning period had significantly higher quality than those collected at the beginning and the end of the spawning period in terms of plasma membrane integrity, MMP, and AI of fresh and post-thaw sperm, post-thaw sperm motility, and fertilization rate. No significant difference was found between 2- and 3-yr-old animals in most quality parameters evaluated. The results suggested that sperm collected during the middle of the spawning season should be used for cryopreservation. The efficiency of genetic improvement programs can be further enhanced by using sperm collected from 2-yr-old abalone which has a similar ability to tolerate cryopreservation as 3-yr-old counterparts.

    Original languageEnglish
    Pages (from-to)665-671
    Number of pages7
    JournalJournal of The World Aquaculture Society
    Volume46
    Issue number6
    DOIs
    Publication statusPublished - 1 Dec 2015

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