Effects of Calcium and Vanadium Ions on the Epoxidation of Cyclohexene with H2O2 Catalyzed by Vanadium Bromoperoxidase

Biaoming Zhang, Xupeng Cao, S Xue, Tonghu Xiao, Wei Zhang

    Research output: Contribution to journalArticle

    Abstract

    Vanadium bromoperoxidase (V-BPO) from a marine macro-alga Corallina officinalis can catalyze the epoxidation of cyclohexene in the presence of KBr to form epoxycyclohexane using H 2 O 2 as the oxidant. A process intensification strategy has been developed by the integration of V-BPO pretreatment, continuous feeding of H 2 O 2 , and optimizing reaction conditions in order to improve the epoxidation productivity. The BPO activity and thermostability increased significantly when the V-BPO solution was dialyzed with a buffer containing 1.0 mmol/L vanadium ion and 1.0 mmol/L calcium ion. The apparent enzyme activity was improved when the H 2 O 2 was fed continuously using a constant flow pump. When combined with the optimal reaction conditions (including buffer, pH, temperature, enzyme concentration, and KBr and H 2 O 2 concentrations), the space-time yield of epoxycyclohexane and its yield to hydrogen peroxide reached 4.79 g/(L · h) and 74%, respectively. These values are 78% increasing than the highest values reported previously.

    Original languageEnglish
    Pages (from-to)1293-1299
    Number of pages7
    JournalChinese Journal of Catalysis
    Volume31
    Issue number10
    DOIs
    Publication statusPublished - Oct 2010

    Keywords

    • Bromoperoxidase
    • Calcium ion
    • Cyclohexene
    • Epoxidation
    • Epoxycyclohexane
    • Hydrogen peroxide
    • Vanadium ion

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