Abstract
Vanadium bromoperoxidase (V-BPO) from a marine macro-alga Corallina officinalis can catalyze the epoxidation of cyclohexene in the presence of KBr to form epoxycyclohexane using H 2 O 2 as the oxidant. A process intensification strategy has been developed by the integration of V-BPO pretreatment, continuous feeding of H 2 O 2 , and optimizing reaction conditions in order to improve the epoxidation productivity. The BPO activity and thermostability increased significantly when the V-BPO solution was dialyzed with a buffer containing 1.0 mmol/L vanadium ion and 1.0 mmol/L calcium ion. The apparent enzyme activity was improved when the H 2 O 2 was fed continuously using a constant flow pump. When combined with the optimal reaction conditions (including buffer, pH, temperature, enzyme concentration, and KBr and H 2 O 2 concentrations), the space-time yield of epoxycyclohexane and its yield to hydrogen peroxide reached 4.79 g/(L · h) and 74%, respectively. These values are 78% increasing than the highest values reported previously.
Original language | English |
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Pages (from-to) | 1293-1299 |
Number of pages | 7 |
Journal | Chinese Journal of Catalysis |
Volume | 31 |
Issue number | 10 |
DOIs | |
Publication status | Published - Oct 2010 |
Keywords
- Bromoperoxidase
- Calcium ion
- Cyclohexene
- Epoxidation
- Epoxycyclohexane
- Hydrogen peroxide
- Vanadium ion