Efficient discovery of immune response targets by cyclical refinement of QSAR models of peptide binding

Vladimir Brusic, Kim Bucci, Christian Schönbach, Nikolai Petrovsky, John Zeleznikow, James W. Kazura

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Peptides that induce and recall T-cell responses are called T-cell epitopes. T-cell epitopes may be useful in a subunit vaccine against malaria. Computer models that simulate peptide binding to MHC are useful for selecting candidate T-cell epitopes since they minimize the number of experiments required for their identification. We applied a combination of computational and immunological strategies to select candidate T-cell epitopes. A total of 86 experimental binding assays were performed in three rounds of identification of HLA-A11 binding peptides from the six pre-erythrocytic malaria antigens. Thirty-six peptides were experimentally confirmed as binders. We show that the cyclical refinement of the ANN models results in a significant improvement of the efficiency of identifying potential T-cell epitopes.

Original languageEnglish
Pages (from-to)405-411
Number of pages7
JournalJournal of Molecular Graphics and Modelling
Volume19
Issue number5
DOIs
Publication statusPublished - Oct 2001
Externally publishedYes

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