Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis

Biaoming Zhang, Xupeng Cao, Xiaofei Cheng, Peichun Wu, Tonghu Xiao, Wei Zhang

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70°C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.

    Original languageEnglish
    Pages (from-to)545-548
    Number of pages4
    JournalBiotechnology Letters
    Volume33
    Issue number3
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Corallina officinalis
    • Heat treatment
    • Protein purification
    • Vanadium-dependent bromoperoxidase

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