TY - JOUR
T1 - Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis
AU - Zhang, Biaoming
AU - Cao, Xupeng
AU - Cheng, Xiaofei
AU - Wu, Peichun
AU - Xiao, Tonghu
AU - Zhang, Wei
PY - 2011/3
Y1 - 2011/3
N2 - A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70°C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.
AB - A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70°C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.
KW - Corallina officinalis
KW - Heat treatment
KW - Protein purification
KW - Vanadium-dependent bromoperoxidase
UR - http://www.scopus.com/inward/record.url?scp=79951772398&partnerID=8YFLogxK
U2 - 10.1007/s10529-010-0454-y
DO - 10.1007/s10529-010-0454-y
M3 - Article
SN - 0141-5492
VL - 33
SP - 545
EP - 548
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 3
ER -