Electrochemistry-enabled fabrication of orthogonal nanotopography and surface chemistry gradients for high-throughput screening

Lauren Clements, Peng-Yuan Wang, Wei-Bor Tsai, Helmut Thissen, Nicolas Voelcker

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Gradient surfaces are emerging tools for investigating mammalian cell-surface interactions in high throughput. We demonstrate the electrochemical fabrication of an orthogonal gradient platform combining a porous silicon (pSi) pore size gradient with an orthogonal gradient of peptide ligand density. pSi gradients were fabricated via the anodic etching of a silicon wafer with pore sizes ranging from hundreds to tens of nanometers. A chemical gradient of ethyl-6-bromohexanoate was generated orthogonally to the pSi gradient via electrochemical attachment. Subsequent hydrolysis and activation of the chemical gradient allowed for the generation of a cyclic RGD gradient. Whilst mesenchymal stem cells (MSC) were shown to respond to both the topographical and chemical cues arising from the orthogonal gradient, the MSC's responded more strongly to changes in RGD density than to changes in pore size during short-term culture.

    Original languageEnglish
    Pages (from-to)1480-1486
    Number of pages7
    JournalLab on a Chip
    Volume12
    Issue number8
    DOIs
    Publication statusPublished - 2012

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