Electrophysiology of guinea-pig myenteric neurons correlated with immunoreactivity for calcium binding proteins

V. Iyer, J. C. Bornstein, M. Costa, J. B. Furness, Y. Takahashi, T. Iwanaga

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    Abstract

    Experiments were undertaken to define the electrophysiological characteristics and shapes of neurons in the myenteric plexus of the guinea-pig ileum that are immunoreactive for calcium binding proteins. Recordings were made from the neurons with intracellular microelectrodes containing a mixture of the fluorescent dye Lucifer yellow and KCl solution. The neurons studied were filled with Lucifer yellow so that they could be re-identified after processing the tissue to reveal immunoreactivity for either the calcium binding protein (CaBP), spot 35 protein, or vitamin D-dependent CaBP. Neurons were characterized as being AH-neurons, in which each action potential is followed by a prolonged after-hyperpolarization (> 4 s), or S-neurons, in which the prolonged after-hyperpolarizations were not observed and focal stimulation of internodal strands evoked fast excitatory synaptic potentials. S-neurons were never immunoreactive for the CaBPs (108 cells), but most AH-neurons (62 of 74) were immunoreactive. Immunoreactive and non-immunoreactive AH-neurons were indistinguishable on the basis of their electrophysiological properties or their shapes (all the AH-neurons were Dogiel type II in shape, i.e. smooth soma and many long processes). The S-neurons had a variety of shapes, but none could be classified as Dogiel type II. It is concluded that most AH-neurons are immunoreactive for calcium binding proteins, and that these proteins are restricted to AH-neurons.

    Original languageEnglish
    Pages (from-to)141-150
    Number of pages10
    JournalJournal of the Autonomic Nervous System
    Volume22
    Issue number2
    DOIs
    Publication statusPublished - Mar 1988

    Keywords

    • AH-neuron
    • Calcium-binding protein
    • Myenteric plexus

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