Encompassing new use cases - level 3.0 of the HUPO-PSI format for molecular interactions

Marine Dumousseau, Diego Alonso-Lopez, Mais Ammari, Glyn Bradley, Nancy Campbell, Arnaud Ceol, Gianni Cesareni, Colin Combe, Javier De Las Rivas, Noemi Del-Toro, Joshua Heimbach, Henning Hermjakob, Igor Jurisica, Maximilian Koch, Luana Licata, Ruth Lovering, David Lynn, Birgit Meldal, Gos Micklem, Simona PanniPablo Porras, Sylvie Ricard-Blum, Bernd Roechert, Lukasz Salwinska, Abhinav Shrivastava, Julie Sullivan, Nicolas Thierry-Mieg, Yo Yehudi, Kim Van Roey, Sandra Orchard

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)
9 Downloads (Pure)


Background: Systems biologists study interaction data to understand the behaviour of whole cell systems, and their environment, at a molecular level. In order to effectively achieve this goal, it is critical that researchers have high quality interaction datasets available to them, in a standard data format, and also a suite of tools with which to analyse such data and form experimentally testable hypotheses from them. The PSI-MI XML standard interchange format was initially published in 2004, and expanded in 2007 to enable the download and interchange of molecular interaction data. PSI-XML2.5 was designed to describe experimental data and to date has fulfilled this basic requirement. However, new use cases have arisen that the format cannot properly accommodate. These include data abstracted from more than one publication such as allosteric/cooperative interactions and protein complexes, dynamic interactions and the need to link kinetic and affinity data to specific mutational changes. Results: The Molecular Interaction workgroup of the HUPO-PSI has extended the existing, well-used XML interchange format for molecular interaction data to meet new use cases and enable the capture of new data types, following extensive community consultation. PSI-MI XML3.0 expands the capabilities of the format beyond simple experimental data, with a concomitant update of the tool suite which serves this format. The format has been implemented by key data producers such as the International Molecular Exchange (IMEx) Consortium of protein interaction databases and the Complex Portal. Conclusions: PSI-MI XML3.0 has been developed by the data producers, data users, tool developers and database providers who constitute the PSI-MI workgroup. This group now actively supports PSI-MI XML2.5 as the main interchange format for experimental data, PSI-MI XML3.0 which additionally handles more complex data types, and the simpler, tab-delimited MITAB2.5, 2.6 and 2.7 for rapid parsing and download.

Original languageEnglish
Article number134
Number of pages8
JournalBMC Bioinformatics
Issue number1
Publication statusPublished - 11 Apr 2018


  • Data standards
  • Molecular interactions
  • Protein complexes
  • Protein-protein interaction
  • PSI-MI
  • XML


Dive into the research topics of 'Encompassing new use cases - level 3.0 of the HUPO-PSI format for molecular interactions'. Together they form a unique fingerprint.

Cite this