Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection

Sara De Biasi; Domenico  Lo Tartaro; Lara  Gibellini; Annamaria  Paolini; Andrew  Quong; Carlene Petes; Geneve Awong; Samuel Douglas; Dongxia Lin; Jordan Nieto; Francesco M Galassi; Rebecca Borella; Lucia Fidanza; Marco Mattioli; Chiara  Leone; Isabella Neri; Marianna  Meschiari; Luca  Cicchetti; Anna G. Iannone; Tommaso Trenti; Mario Sarti; Massimo Girardis; Giovanni  Guaraldi; Cristina Mussini; Fabio  Facchinetti; Andrea Cossarizza

doi:10.1038/s41467-021-24940-w

Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection

Sara De Biasi, Domenico Lo Tartaro, Lara Gibellini, Annamaria Paolini, Andrew Quong, Carlene Petes, Geneve Awong, Samuel Douglas, Dongxia Lin, Jordan Nieto, Francesco M Galassi, Rebecca Borella, Lucia Fidanza, Marco Mattioli, Chiara Leone, Isabella Neri, Marianna Meschiari, Luca Cicchetti, Anna G. Iannone, Tommaso TrentiMario Sarti, Massimo Girardis, Giovanni Guaraldi, Cristina Mussini, Fabio Facchinetti, Andrea Cossarizza

College of Humanities, Arts and Social Sciences

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Abstract

SARS-CoV-2 infection can affect all human beings, including pregnant women. Thus, understanding the immunological changes induced by the virus during pregnancy is nowadays of pivotal importance. Here, using peripheral blood from 14 pregnant women with asymptomatic or mild SARS-CoV-2 infection, we investigate cell proliferation and cytokine production, measure plasma levels of 62 cytokines, and perform a 38-parameter mass cytometry analysis. Our results show an increase in low density neutrophils but no lymphopenia or gross alterations of white blood cells, which display normal levels of differentiation, activation or exhaustion markers and show well preserved functionality. Meanwhile, the plasma levels of anti-inflammatory cytokines such as interleukin (IL)-1RA, IL-10 and IL-19 are increased, those of IL-17, PD-L1 and D-dimer are decreased, but IL-6 and other inflammatory molecules remain unchanged. Our profiling of antiviral immune responses may thus help develop therapeutic strategies to avoid virus-induced damages during pregnancy.

Original language	English
Article number	4677
Pages (from-to)	1-16
Number of pages	16
Journal	Nature Communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-24940-w
Publication status	Published - 29 Jul 2021

Keywords

SARS-Cov-2
Pregnancy
Immunology
inflammation
asymptomatic
cytokines
IL-17 signaling pathway
Neutrophils
translational immunology
viral infection

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De Biasi, S., Lo Tartaro, D., Gibellini, L., Paolini, A., Quong, A., Petes, C., Awong, G., Douglas, S., Lin, D., Nieto, J., Galassi, F. M., Borella, R., Fidanza, L., Mattioli, M., Leone, C., Neri, I., Meschiari, M., Cicchetti, L., Iannone, A. G., ... Cossarizza, A. (2021). Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection. Nature Communications, 12(1), 1-16. [4677]. https://doi.org/10.1038/s41467-021-24940-w

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title = "Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection",

abstract = "SARS-CoV-2 infection can affect all human beings, including pregnant women. Thus, understanding the immunological changes induced by the virus during pregnancy is nowadays of pivotal importance. Here, using peripheral blood from 14 pregnant women with asymptomatic or mild SARS-CoV-2 infection, we investigate cell proliferation and cytokine production, measure plasma levels of 62 cytokines, and perform a 38-parameter mass cytometry analysis. Our results show an increase in low density neutrophils but no lymphopenia or gross alterations of white blood cells, which display normal levels of differentiation, activation or exhaustion markers and show well preserved functionality. Meanwhile, the plasma levels of anti-inflammatory cytokines such as interleukin (IL)-1RA, IL-10 and IL-19 are increased, those of IL-17, PD-L1 and D-dimer are decreased, but IL-6 and other inflammatory molecules remain unchanged. Our profiling of antiviral immune responses may thus help develop therapeutic strategies to avoid virus-induced damages during pregnancy.",

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author = "{De Biasi}, Sara and {Lo Tartaro}, Domenico and Lara Gibellini and Annamaria Paolini and Andrew Quong and Carlene Petes and Geneve Awong and Samuel Douglas and Dongxia Lin and Jordan Nieto and Galassi, {Francesco M} and Rebecca Borella and Lucia Fidanza and Marco Mattioli and Chiara Leone and Isabella Neri and Marianna Meschiari and Luca Cicchetti and Iannone, {Anna G.} and Tommaso Trenti and Mario Sarti and Massimo Girardis and Giovanni Guaraldi and Cristina Mussini and Fabio Facchinetti and Andrea Cossarizza",

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day = "29",

doi = "10.1038/s41467-021-24940-w",

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De Biasi, S, Lo Tartaro, D, Gibellini, L, Paolini, A, Quong, A, Petes, C, Awong, G, Douglas, S, Lin, D, Nieto, J, Galassi, FM, Borella, R, Fidanza, L, Mattioli, M, Leone, C, Neri, I, Meschiari, M, Cicchetti, L, Iannone, AG, Trenti, T, Sarti, M, Girardis, M, Guaraldi, G, Mussini, C, Facchinetti, F & Cossarizza, A 2021, 'Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection', Nature Communications, vol. 12, no. 1, 4677, pp. 1-16. https://doi.org/10.1038/s41467-021-24940-w

TY - JOUR

T1 - Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection

AU - De Biasi, Sara

AU - Lo Tartaro, Domenico

AU - Gibellini, Lara

AU - Paolini, Annamaria

AU - Quong, Andrew

AU - Petes, Carlene

AU - Awong, Geneve

AU - Douglas, Samuel

AU - Lin, Dongxia

AU - Nieto, Jordan

AU - Galassi, Francesco M

AU - Borella, Rebecca

AU - Fidanza, Lucia

AU - Mattioli, Marco

AU - Leone, Chiara

AU - Neri, Isabella

AU - Meschiari, Marianna

AU - Cicchetti, Luca

AU - Iannone, Anna G.

AU - Trenti, Tommaso

AU - Sarti, Mario

AU - Girardis, Massimo

AU - Guaraldi, Giovanni

AU - Mussini, Cristina

AU - Facchinetti, Fabio

AU - Cossarizza, Andrea

PY - 2021/7/29

Y1 - 2021/7/29

N2 - SARS-CoV-2 infection can affect all human beings, including pregnant women. Thus, understanding the immunological changes induced by the virus during pregnancy is nowadays of pivotal importance. Here, using peripheral blood from 14 pregnant women with asymptomatic or mild SARS-CoV-2 infection, we investigate cell proliferation and cytokine production, measure plasma levels of 62 cytokines, and perform a 38-parameter mass cytometry analysis. Our results show an increase in low density neutrophils but no lymphopenia or gross alterations of white blood cells, which display normal levels of differentiation, activation or exhaustion markers and show well preserved functionality. Meanwhile, the plasma levels of anti-inflammatory cytokines such as interleukin (IL)-1RA, IL-10 and IL-19 are increased, those of IL-17, PD-L1 and D-dimer are decreased, but IL-6 and other inflammatory molecules remain unchanged. Our profiling of antiviral immune responses may thus help develop therapeutic strategies to avoid virus-induced damages during pregnancy.

AB - SARS-CoV-2 infection can affect all human beings, including pregnant women. Thus, understanding the immunological changes induced by the virus during pregnancy is nowadays of pivotal importance. Here, using peripheral blood from 14 pregnant women with asymptomatic or mild SARS-CoV-2 infection, we investigate cell proliferation and cytokine production, measure plasma levels of 62 cytokines, and perform a 38-parameter mass cytometry analysis. Our results show an increase in low density neutrophils but no lymphopenia or gross alterations of white blood cells, which display normal levels of differentiation, activation or exhaustion markers and show well preserved functionality. Meanwhile, the plasma levels of anti-inflammatory cytokines such as interleukin (IL)-1RA, IL-10 and IL-19 are increased, those of IL-17, PD-L1 and D-dimer are decreased, but IL-6 and other inflammatory molecules remain unchanged. Our profiling of antiviral immune responses may thus help develop therapeutic strategies to avoid virus-induced damages during pregnancy.

KW - SARS-Cov-2

KW - Pregnancy

KW - Immunology

KW - inflammation

KW - asymptomatic

KW - cytokines

KW - IL-17 signaling pathway

KW - Neutrophils

KW - translational immunology

KW - viral infection

UR - http://www.scopus.com/inward/record.url?scp=85111480124&partnerID=8YFLogxK

U2 - 10.1038/s41467-021-24940-w

DO - 10.1038/s41467-021-24940-w

M3 - Article

VL - 12

SP - 1

EP - 16

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 4677

ER -

Endogenous control of inflammation characterizes pregnant women with asymptomatic or paucisymptomatic SARS-CoV-2 infection

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Keywords

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