Endothelial progenitor cells enhance islet engraftment, influence β-cell function, and modulate islet connexin 36 expression

Daniella Penko, Darling Rojas-Canales, Daisy Mohanasundaram, Galhenage Peiris, Wai Sun, Chris Drogemuller, Damien Keating, P Coates, Claudine Bonder, Claire Jessup

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)

    Abstract

    The success of pancreatic islet transplantation is limited by delayed engraftment and suboptimal function in the longer term. Endothelial progenitor cells (EPCs) represent a potential cellular therapy that may improve the engraftment of transplanted pancreatic islets. In addition, EPCs may directly affect the function of pancreatic β-cells. The objective of this study was to examine the ability of EPCs to enhance pancreatic islet transplantation in a murine syngeneic marginal mass transplant model and to examine the mechanisms through which this occurs. We found that cotransplanted EPCs improved the cure rate and initial glycemic control of transplanted islets. Gene expression data indicate that EPCs, or their soluble products, modulate the expression of the β-cell surface molecule connexin 36 and affect glucose-stimulated insulin release in vitro. In conclusion, EPCs are a promising candidate for improving outcomes in islet transplantation, and their mechanisms of action warrant further study.

    Original languageEnglish
    Pages (from-to)37-48
    Number of pages12
    JournalCELL TRANSPLANTATION
    Volume24
    Issue number1
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Cell therapy
    • Connexin 36
    • Diabetes
    • Endothelial progenitor cells (EPCs)
    • Islet transplantation
    • Islets of Langerhans

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