Environmental stresses inhibit and stimulate different protein import pathways in plant mitochondria

Nicolas L. Taylor, Charlotta Rudhe, Joanne M. Hulett, Trevor Lithgow, Elzbieta Glaser, David A. Day, A. Harvey Millar, James Whelan

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

The impact of various environmental stresses (drought, chilling or herbicide treatment) on the capacity of plant mitochondria to import precursor proteins was investigated. Drought treatment stimulated import and processing of various precursor proteins via the general import pathway. The stimulatory effect of drought on the general import pathway was due to an increased rate of import, was accompanied by an increased rate of processing, and could be attributed to the presequence of the precursor protein. Interestingly, drought decreased the import of the FAd subunit of ATP synthase suggesting a bypass of the point of stimulation during import of this precursor. Both chilling and herbicide treatment of plants, on the other hand, caused inhibition of import with all precursors tested. No decrease in processing of imported proteins was observed by these stress treatments. Western analysis of several mitochondrial proteins indicated that the steady-state level of several mitochondrial components, including the TOM20 receptor and the core subunits of the cytochrome bc1 complex responsible for processing, remained largely unchanged. Thus environmental stresses differentially affect import of precursor proteins in a complicated manner dependent on the import pathway utilised.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalFEBS Letters
Volume547
Issue number1-3
DOIs
Publication statusPublished - 17 Jul 2003
Externally publishedYes

Keywords

  • Environmental stress
  • Import pathway
  • Mitochondrial import
  • Processing

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