Epiallele quantification using molecular inversion probes.

Ramkumar Palanisamy; Ashley Connolly; Matt Trau

doi:10.1021/ac103016n

Epiallele quantification using molecular inversion probes.

Ramkumar Palanisamy
, Ashley Connolly
, Matt Trau

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The location and level of DNA methylation within a genome is emerging as an important biomarker for cancer diagnosis. Despite its potential, it is difficult to comprehensively analyze the epialleles that are often found in a biological sample. Therefore, an assay utilizing molecular inversion probes was designed and used to expose and quantify epialleles in heterogeneously methylated bisulphite treated genomic DNA. Different CpG dinucleotides were able to be rapidly quantified with high resolution, sensitivity and specificity over a large dynamic range using rapid flow cytometric readout of multiplexable microbead DNA biosensors.

Original language	English
Pages (from-to)	2631-2637
Number of pages	7
Journal	Analytical Chemistry
Volume	83
Issue number	7
DOIs	https://doi.org/10.1021/ac103016n
Publication status	Published - 1 Apr 2011

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10.1021/ac103016n

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abstract = "The location and level of DNA methylation within a genome is emerging as an important biomarker for cancer diagnosis. Despite its potential, it is difficult to comprehensively analyze the epialleles that are often found in a biological sample. Therefore, an assay utilizing molecular inversion probes was designed and used to expose and quantify epialleles in heterogeneously methylated bisulphite treated genomic DNA. Different CpG dinucleotides were able to be rapidly quantified with high resolution, sensitivity and specificity over a large dynamic range using rapid flow cytometric readout of multiplexable microbead DNA biosensors.",

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AB - The location and level of DNA methylation within a genome is emerging as an important biomarker for cancer diagnosis. Despite its potential, it is difficult to comprehensively analyze the epialleles that are often found in a biological sample. Therefore, an assay utilizing molecular inversion probes was designed and used to expose and quantify epialleles in heterogeneously methylated bisulphite treated genomic DNA. Different CpG dinucleotides were able to be rapidly quantified with high resolution, sensitivity and specificity over a large dynamic range using rapid flow cytometric readout of multiplexable microbead DNA biosensors.

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JO - Analytical Chemistry

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Epiallele quantification using molecular inversion probes.

Abstract

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