Epidermal growth factor and fluid and electrolyte balance in the rat

Sharon L. Grant, Christine B. Gow, Paddy A. Phillips

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) has been shown to induce a renal diuresis and natriuresis in sheep and stimulate prostaglandin synthesis from inner rat medullary collecting duct cells in culture. The aims of our study were 1) to investigate whether the renal effects of intravenous infusion of EGF were species specific and 2) to determine the mechanism of these effects by studying the interaction between EGF and indomethacin (a prostaglandin synthase inhibitor) in the conscious rat. Sprague-Dawley rats received intravenous infusions of either 0.9% saline or 0.2 or 2.0 μg EGF · kg-1 · h-1 over a 6-day period after an initial baseline period. Infusion of 2.0 μg EGF · kg-1 · h-1 caused an increase in urine volume (baseline: 5.5 ± 0.2 ml to day 5: 9.0 ± 0.4 ml, P < 0.01) and corresponding polydipsia, but not natriuresis. Administration of indomethacin with 2.0 μg EGF · kg-1 · h-1 attenuated (P < 0.05) the diuretic (day 5 EGF + vehicle: 12.2 ± 1.1 ml vs. EGF + indomethacin: 8.7 ± 0.9 ml) and polydipsic effects of EGF. These studies demonstrate that intravenous infusion of EGF causes a diuretic effect in rats without natriuresis and that prostaglandins play a role in the diuretic effect of EGF in the rat.

Original languageEnglish
Pages (from-to)R1853-R1861
Number of pages9
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume272
Issue number6 41-6
DOIs
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • Diuresis
  • Indomethacin
  • Polydipsia

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