Epigenetic Repression of SATB1 by Polycomb Group Protein EZH2 in Epithelial Cells

Lei Li, Lu Lu, Xue-song Wu, De-pei Liu, Chih-chuan Liang

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Objective: To study the regulatory mechanism of SATB1 repression in cells other than T cells or erythroid cells, which have high expression level of SATB1. Methods: HeLa epithelial cells were treated with either histone deacetylase inhibitor (HDACi) trichostatin A (TSA) or DNA methylation inhibitor 5-Aza-C before detecting SATB1 expression. Luciferase reporter system was applied to measure effects of EZH2 on SATB1 promoter activity. Over-expression or knockdown of EZH2 and subsequent quantitative reverse transcription- polymerase chain reaction were performed to determine the effect of this Polycomb group protein on SATB1 transcription. Chromatin immunoprecipitation (ChIP) assay was applied to measure enrichment of EZH2 and trimethylated H3K27 (H3K27me3) at SATB1 promoter in HeLa cells. K562 cells and Jurkat cells, both having high-level expression of SATB1, were used in the ChIP experiment as controls. Results: Both TSA and 5-Aza-C increased SATB1 expression in HeLa cells. Over-expression of EZH2 reduced promoter activity as well as the mRNA level of SATB1, while knockdown of EZH2 apparently enhanced SATB1 expression in HeLa cells but not in K562 cells and Jurkat cells. ChIP assay results suggested that epigenetic silencing of SATB1 by EZH2 in HeLa cells was mediated by trimethylation modification of H3K27. In contrast, enrichment of EZH2 and H3K27me3 was not detected within proximal promoter region of SATB1 in either K562 or Jurkat cells. Conclusion: SATB1 is a bona fide EZH2 target gene in HeLa cells and the repression of SATB1 by EZH2 may be mediated by trimethylation modification on H3K27.

    Original languageEnglish
    Pages (from-to)199-205
    Number of pages7
    JournalCHINESE MEDICAL SCIENCES JOURNAL
    Volume25
    Issue number4
    DOIs
    Publication statusPublished - Dec 2010

    Keywords

    • EZH2
    • Gene silencing
    • Polycomb group protein
    • SATB1
    • Trimethylated H3K27

    Fingerprint Dive into the research topics of 'Epigenetic Repression of SATB1 by Polycomb Group Protein EZH2 in Epithelial Cells'. Together they form a unique fingerprint.

  • Cite this