Establishment of a continuous cell line from southern bluefin tuna (Thunnus maccoyii)

Peter Bain; Rhonda Hutchinson; Alexandra Marks; Mark Crane; Kathryn Schuller

doi:10.1016/j.aquaculture.2012.11.008

Establishment of a continuous cell line from southern bluefin tuna (Thunnus maccoyii)

Peter Bain, Rhonda Hutchinson, Alexandra Marks, Mark Crane, Kathryn Schuller

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A continuous cell line, designated SBT-E1, was established from tissues of a southern bluefin tuna (Thunnus maccoyii) fingerling. Maximum cell proliferation rates occurred at 25 °C in Liebovitz's L-15 medium containing 10-20% FBS. Survival of SBT-E1 after short-term cryostorage was 70.8 ± 12.8%. The species of origin was confirmed by sequencing a region of the mitochondrial gene for cytochrome c oxidase, subunit I (cox1). The SBT-E1 karyotype, with a modal chromosome number of 2n = 46, differs from the constitutional karyotype of the parent species (2n = 48), with the apparent loss of either whole or partial chromosomes and the presence of small marker chromosomes. The SBT-E1 cell line represents the first reported cell line from any species of tuna.

Original language	English
Pages (from-to)	59-63
Number of pages	5
Journal	Aquaculture
Volume	376-379
DOIs	https://doi.org/10.1016/j.aquaculture.2012.11.008
Publication status	Published - 5 Feb 2013

Keywords

Cell line
Karyotype
Southern bluefin tuna
Thunnus maccoyii

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title = "Establishment of a continuous cell line from southern bluefin tuna (Thunnus maccoyii)",

abstract = "A continuous cell line, designated SBT-E1, was established from tissues of a southern bluefin tuna (Thunnus maccoyii) fingerling. Maximum cell proliferation rates occurred at 25 °C in Liebovitz's L-15 medium containing 10-20% FBS. Survival of SBT-E1 after short-term cryostorage was 70.8 ± 12.8%. The species of origin was confirmed by sequencing a region of the mitochondrial gene for cytochrome c oxidase, subunit I (cox1). The SBT-E1 karyotype, with a modal chromosome number of 2n = 46, differs from the constitutional karyotype of the parent species (2n = 48), with the apparent loss of either whole or partial chromosomes and the presence of small marker chromosomes. The SBT-E1 cell line represents the first reported cell line from any species of tuna.",

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author = "Peter Bain and Rhonda Hutchinson and Alexandra Marks and Mark Crane and Kathryn Schuller",

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doi = "10.1016/j.aquaculture.2012.11.008",

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T1 - Establishment of a continuous cell line from southern bluefin tuna (Thunnus maccoyii)

AU - Bain, Peter

AU - Hutchinson, Rhonda

AU - Marks, Alexandra

AU - Crane, Mark

AU - Schuller, Kathryn

PY - 2013/2/5

Y1 - 2013/2/5

N2 - A continuous cell line, designated SBT-E1, was established from tissues of a southern bluefin tuna (Thunnus maccoyii) fingerling. Maximum cell proliferation rates occurred at 25 °C in Liebovitz's L-15 medium containing 10-20% FBS. Survival of SBT-E1 after short-term cryostorage was 70.8 ± 12.8%. The species of origin was confirmed by sequencing a region of the mitochondrial gene for cytochrome c oxidase, subunit I (cox1). The SBT-E1 karyotype, with a modal chromosome number of 2n = 46, differs from the constitutional karyotype of the parent species (2n = 48), with the apparent loss of either whole or partial chromosomes and the presence of small marker chromosomes. The SBT-E1 cell line represents the first reported cell line from any species of tuna.

AB - A continuous cell line, designated SBT-E1, was established from tissues of a southern bluefin tuna (Thunnus maccoyii) fingerling. Maximum cell proliferation rates occurred at 25 °C in Liebovitz's L-15 medium containing 10-20% FBS. Survival of SBT-E1 after short-term cryostorage was 70.8 ± 12.8%. The species of origin was confirmed by sequencing a region of the mitochondrial gene for cytochrome c oxidase, subunit I (cox1). The SBT-E1 karyotype, with a modal chromosome number of 2n = 46, differs from the constitutional karyotype of the parent species (2n = 48), with the apparent loss of either whole or partial chromosomes and the presence of small marker chromosomes. The SBT-E1 cell line represents the first reported cell line from any species of tuna.

KW - Cell line

KW - Karyotype

KW - Southern bluefin tuna

KW - Thunnus maccoyii

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DO - 10.1016/j.aquaculture.2012.11.008

M3 - Article

VL - 376-379

SP - 59

EP - 63

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -

Establishment of a continuous cell line from southern bluefin tuna (Thunnus maccoyii)

Abstract

Keywords

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