Evaluation and Optimizations of Media on the Tissue Culture System of Upland Rice

Mature seeds of Malaysian upland rice, cv. Selasi were selected as starting material for callus production and regeneration response in serial experiments. Four media, three carbon sources at three concentrations and four gelrite concentrations were tested. Moreover, different concentrations of proline, casein hydrolysate and sorbitol were also examined. The results obtained from our study suggested that the optimal callus induction medium was MSB5 supplemented with 2 mg L^-1 2, 4-D (previous work, Shahsavari et al., 2010), 40 g L^-1 maltose, 600 mg L^-1 casein hydrolysate and 0.4% gelrite. While maximum regeneration response was detected on MSB5 medium fortified with 0.5 mg L^-1 NAA+2.0 mg L^-1 Kin+2.0 mg L^-1 BAP, 30 g L^-1 maltose or 30 g L^-1 sucrose plus 10 g L^-1 sorbitol, 600 mg L^-1casein hydrolysate, which was solidified with 0.4% gelrite.