Evaluation of cell disruption method for lipase extraction from novel thraustochytrids

Avinesh Byreddy; Nalam Rao; Colin Barrow; Munish Puri

doi:10.1016/j.algal.2017.04.025

Evaluation of cell disruption method for lipase extraction from novel thraustochytrids

Avinesh Byreddy, Nalam Rao, Colin Barrow, Munish Puri

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Marine microorganisms represent a rich source of enzymes with the potential industrial application. In this study, we screened novel thraustochytrids for a range of enzymatic activities. The similarity between isolates based on enzyme activities was investigated using cluster analysis. Thraustochytrids were observed to possess different enzymatic activities, however, we focused on lipase activity. Two strains AMCQ-4b27 (34 IU/g) and AMCQS1-9 (36 IU/g) exhibited highest intracellular lipase activity among the isolates. To extract lipase, four different cell disruption methods such as bead vortexing, grinding, sonication and homogenization, were evaluated for their efficiency on lipase extraction yields and quality. Sonication was found to be the best method for enhancing lipase extraction yields from Thraustochytrium sp. AMCQ-4b27 (0.903 IU/μg) and Schizochytrium AMCQS1-9 (1.44 IU/μg).

Original language	English
Pages (from-to)	62-67
Number of pages	6
Journal	Algal Research
Volume	25
DOIs	https://doi.org/10.1016/j.algal.2017.04.025
Publication status	Published - 1 Jul 2017

Keywords

Cell disruption
Cluster analysis
Marine
Omega-3 fatty acids
Sonication
Thraustochytrium

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.algal.2017.04.025

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title = "Evaluation of cell disruption method for lipase extraction from novel thraustochytrids",

abstract = "Marine microorganisms represent a rich source of enzymes with the potential industrial application. In this study, we screened novel thraustochytrids for a range of enzymatic activities. The similarity between isolates based on enzyme activities was investigated using cluster analysis. Thraustochytrids were observed to possess different enzymatic activities, however, we focused on lipase activity. Two strains AMCQ-4b27 (34 IU/g) and AMCQS1-9 (36 IU/g) exhibited highest intracellular lipase activity among the isolates. To extract lipase, four different cell disruption methods such as bead vortexing, grinding, sonication and homogenization, were evaluated for their efficiency on lipase extraction yields and quality. Sonication was found to be the best method for enhancing lipase extraction yields from Thraustochytrium sp. AMCQ-4b27 (0.903 IU/μg) and Schizochytrium AMCQS1-9 (1.44 IU/μg).",

keywords = "Cell disruption, Cluster analysis, Marine, Omega-3 fatty acids, Sonication, Thraustochytrium",

author = "Avinesh Byreddy and Nalam Rao and Colin Barrow and Munish Puri",

year = "2017",

month = jul,

day = "1",

doi = "10.1016/j.algal.2017.04.025",

language = "English",

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journal = "Algal Research",

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T1 - Evaluation of cell disruption method for lipase extraction from novel thraustochytrids

AU - Byreddy, Avinesh

AU - Rao, Nalam

AU - Barrow, Colin

AU - Puri, Munish

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Marine microorganisms represent a rich source of enzymes with the potential industrial application. In this study, we screened novel thraustochytrids for a range of enzymatic activities. The similarity between isolates based on enzyme activities was investigated using cluster analysis. Thraustochytrids were observed to possess different enzymatic activities, however, we focused on lipase activity. Two strains AMCQ-4b27 (34 IU/g) and AMCQS1-9 (36 IU/g) exhibited highest intracellular lipase activity among the isolates. To extract lipase, four different cell disruption methods such as bead vortexing, grinding, sonication and homogenization, were evaluated for their efficiency on lipase extraction yields and quality. Sonication was found to be the best method for enhancing lipase extraction yields from Thraustochytrium sp. AMCQ-4b27 (0.903 IU/μg) and Schizochytrium AMCQS1-9 (1.44 IU/μg).

AB - Marine microorganisms represent a rich source of enzymes with the potential industrial application. In this study, we screened novel thraustochytrids for a range of enzymatic activities. The similarity between isolates based on enzyme activities was investigated using cluster analysis. Thraustochytrids were observed to possess different enzymatic activities, however, we focused on lipase activity. Two strains AMCQ-4b27 (34 IU/g) and AMCQS1-9 (36 IU/g) exhibited highest intracellular lipase activity among the isolates. To extract lipase, four different cell disruption methods such as bead vortexing, grinding, sonication and homogenization, were evaluated for their efficiency on lipase extraction yields and quality. Sonication was found to be the best method for enhancing lipase extraction yields from Thraustochytrium sp. AMCQ-4b27 (0.903 IU/μg) and Schizochytrium AMCQS1-9 (1.44 IU/μg).

KW - Cell disruption

KW - Cluster analysis

KW - Marine

KW - Omega-3 fatty acids

KW - Sonication

KW - Thraustochytrium

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U2 - 10.1016/j.algal.2017.04.025

DO - 10.1016/j.algal.2017.04.025

M3 - Article

SN - 2211-9264

VL - 25

SP - 62

EP - 67

JO - Algal Research

JF - Algal Research

ER -

Evaluation of cell disruption method for lipase extraction from novel thraustochytrids

Abstract

Keywords

UN SDGs

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