Evaluation of cell disruption method for lipase extraction from novel thraustochytrids

Avinesh Byreddy, Nalam Rao, Colin Barrow, Munish Puri

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    Marine microorganisms represent a rich source of enzymes with the potential industrial application. In this study, we screened novel thraustochytrids for a range of enzymatic activities. The similarity between isolates based on enzyme activities was investigated using cluster analysis. Thraustochytrids were observed to possess different enzymatic activities, however, we focused on lipase activity. Two strains AMCQ-4b27 (34 IU/g) and AMCQS1-9 (36 IU/g) exhibited highest intracellular lipase activity among the isolates. To extract lipase, four different cell disruption methods such as bead vortexing, grinding, sonication and homogenization, were evaluated for their efficiency on lipase extraction yields and quality. Sonication was found to be the best method for enhancing lipase extraction yields from Thraustochytrium sp. AMCQ-4b27 (0.903 IU/μg) and Schizochytrium AMCQS1-9 (1.44 IU/μg).

    Original languageEnglish
    Pages (from-to)62-67
    Number of pages6
    JournalAlgal Research
    Volume25
    DOIs
    Publication statusPublished - 1 Jul 2017

    Keywords

    • Cell disruption
    • Cluster analysis
    • Marine
    • Omega-3 fatty acids
    • Sonication
    • Thraustochytrium

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