TY - JOUR
T1 - Evidence from twins for acquired cellular immune hyperactivity in type 1 diabetes
AU - Petrovsky, Nikolai
AU - Kyvik, Kirsten O.
AU - Bonnevie-Nielsen, Vagn
AU - Beck-Nielsen, Henning
AU - Green, Anders
AU - Harrison, Leonard C.
PY - 2002/8
Y1 - 2002/8
N2 - Type 1 diabetes has been associated with an increased frequency of activated T cells and T-cell hyperactivity to non-specific and disease-specific stimuli including the islet autoantigen glutamic acid decarboxylase 65 (GAD). To address whether T-cell hyperactivity is genetic or acquired we measured whole blood cytokines in vitro in response to GAD or tetanus in 18 identical twin pairs, nine discordant for type 1 diabetes. In addition, the activity of 2′, 5′ oligoadenylate synthetase (OAS) in blood mononuclear cells was measured as a marker of viral infection. Interleukin-2 (IL-2) basally and IL-2 and interferon-γ (IFN-γ) in response to GAD, were detected more frequently and at higher levels in diabetic compared to non-diabetic twins. IL-10 was not different between groups. OAS activity was increased in diabetic compared to non-diabetic twins and showed a correlation with basal IL-2 and GAD-stimulated IFN-γ and IL-10. These findings suggest that T-cell hyperactivity in type 1 diabetes is an acquired trait and could reflect persisting virus expression.
AB - Type 1 diabetes has been associated with an increased frequency of activated T cells and T-cell hyperactivity to non-specific and disease-specific stimuli including the islet autoantigen glutamic acid decarboxylase 65 (GAD). To address whether T-cell hyperactivity is genetic or acquired we measured whole blood cytokines in vitro in response to GAD or tetanus in 18 identical twin pairs, nine discordant for type 1 diabetes. In addition, the activity of 2′, 5′ oligoadenylate synthetase (OAS) in blood mononuclear cells was measured as a marker of viral infection. Interleukin-2 (IL-2) basally and IL-2 and interferon-γ (IFN-γ) in response to GAD, were detected more frequently and at higher levels in diabetic compared to non-diabetic twins. IL-10 was not different between groups. OAS activity was increased in diabetic compared to non-diabetic twins and showed a correlation with basal IL-2 and GAD-stimulated IFN-γ and IL-10. These findings suggest that T-cell hyperactivity in type 1 diabetes is an acquired trait and could reflect persisting virus expression.
UR - http://www.scopus.com/inward/record.url?scp=0036037119&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2567.2002.01449.x
DO - 10.1046/j.1365-2567.2002.01449.x
M3 - Article
C2 - 12153522
AN - SCOPUS:0036037119
SN - 0019-2805
VL - 106
SP - 584
EP - 589
JO - Immunology
JF - Immunology
IS - 4
ER -
