Evidence of multiple forms of rat liver microsomal coenzyme A ligase catalysing the formation of 2-arylpropionyl-coenzyme A thioesters

Kathleen M. Knights, Ursula M. Talbot, Thomas A. Baillie

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    27 Citations (Scopus)

    Abstract

    This study has demonstrated the involvement of multiple forms of rat hepatic microsomal CoA ligases in the formation of 2-arylpropionyl-CoA thioesters. In the presence of (-)R-ibuprofen (0.1 μM-1 mM) two enzymic processes were observed, one of which exhibited enantiospecificity and apparent high affinity for the R enantiomer (Km 0.06 μM) whilst the second, a low-affinity component was non-enantiospecific. An equivalent hi-affinity isoform catalysing R-flurbiprofen-CoA formation at concentrations less than 100 μM was not demonstrated. However, at higher substrate concentrations formation of both R- and S-flurbiprofenyl-CoA thiosters occurred. Marked inter-individual variation was observed in the formation of S-ibuprofen-CoA and S-flurbiprofen-CoA in the rats studied.

    Original languageEnglish
    Pages (from-to)2415-2417
    Number of pages3
    JournalBiochemical Pharmacology
    Volume44
    Issue number12
    DOIs
    Publication statusPublished - 15 Dec 1992

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