Evidence that catecholamine-synthesizing perikarya in rat medulla oblongata do not contribute axons to the vagus nerve

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    Abstract

    We attempted to confirm reports that medullary catecholamine-synthesizing neurons in the rat contribute axons to the vagus nerve. Vagal preganglionic neurons in the medulla were identified by the retrograde intra-axonal transport of Fast Blue from the cervical vagus. Catecholamine-synthesizing neurons were identified using a specific antibody against tyrosine hydroxylase. A rhodamine-labelled second antibody was used to ensure that Fast Blue and tyrosine hydroxylase could be viewed entirely independently. We did not find any medullary neurons which contained both tyrosine hydroxylase and Fast Blue. Although further investigations by other laboratories are necessary, we believe that previous studies, using punctate versus diffuse horseradish peroxidase staining to doubly label neurons may have produced false positive results.

    Original languageEnglish
    Pages (from-to)397-400
    Number of pages4
    JournalBrain Research
    Volume348
    Issue number2
    DOIs
    Publication statusPublished - 2 Dec 1985

    Keywords

    • catecholamine-containing neuron
    • dorsal motor nucleus of the vagus
    • Fast Blue retrograde intra-axonal transport
    • medulla oblongata
    • nucleus ambiguus
    • vagal preganglionic cell

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