Evidence that myoelectric complexes in the isolated mouse colon may not be of myogenic origin

N. J. Spencer, R. A.R. Bywater, G. S. Taylor

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The hypothesis that spontaneous depolarisations (myoelectric complexes, MCs) can occur in the absence of neuronal activity, depending on the level of the membrane potential, was systematically studied. In control Krebs' solution, MCs were recorded approximately every 5 min and were abolished by tetrodotoxin (TTX, 1.6 μM). However, TTX also induced sustained membrane depolarisation (19 mV) in the circular muscle. To test whether MCs were blocked by the depolarisation induced by TTX, graded membrane repolarisations were generated, in the continuing presence of TTX, using sodium nitroprusside (SNP, 10 nM-1 μM). Under these conditions, MC activity was not restored. The addition of SNP (1 μM) to control preparations, in normal Krebs' solution, hyperpolarised the membrane of the circular muscle cells, but did not inhibit ongoing MC activity. It is suggested that the underlying mechanisms involved in MC generation are unlikely to be dependent upon the level of membrane potential in circular smooth muscle.

Original languageEnglish
Pages (from-to)153-156
Number of pages4
JournalNeuroscience letters
Volume250
Issue number3
DOIs
Publication statusPublished - 10 Jul 1998
Externally publishedYes

Keywords

  • Colon
  • Myoelectric complex
  • Tonic neurogenic inhibition

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