Expression and Localization of Homeodomain Proteins DLX4, HB9 and HB24 in Malignant and Benign Human Colorectal Tissues

Paul Hollington, Petra Neufing, Bill Kalionis, Paul Waring, Jacky Bentel, David Wattchow, Wayne D. Tilley

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: The purpose of this study was to identify homeobox genes expressed in the human colon and to determine whether their expression levels were altered between matched non-malignant and malignant colon tissues. Materials and Methods: Homeobox genes expressed in colon tissue were identified by reverse transcription polymerase chain reaction (RT-PCR). Antibodies were raised to the homeodomain proteins DLX4, HB9 and HB24 and immunohistochemistry was performed on 3 moderately-differentiated tumors and their corresponding non-malignant colon tissue samples. Results: The RT-PCR screen identified expression of DLX4, HB9, HB24 and MSX2 in the normal colon. Immunoaffinity purified polyclonal antisera raised against DLX4, HB9 and HB24 detect specific immunoreactivity in glandular epithelial cells, stromal cells of the lamina propria but not in the submucosa. Nuclear epithelial immunoreactivity of all three antibodies decreased in moderately-differentiated tumors compared to the corresponding matched non-malignant mucosa. These data suggest that differential expression of HB9, HB24 and DLX4 may be associated with colorectal carcinogenesis.

Original languageEnglish
Pages (from-to)955-962
Number of pages8
JournalAnticancer Research
Volume24
Issue number2 B
Publication statusPublished - Mar 2004
Externally publishedYes

Keywords

  • Colorectal cancer
  • Homeodomain
  • Immunohistochemistry

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    Hollington, P., Neufing, P., Kalionis, B., Waring, P., Bentel, J., Wattchow, D., & Tilley, W. D. (2004). Expression and Localization of Homeodomain Proteins DLX4, HB9 and HB24 in Malignant and Benign Human Colorectal Tissues. Anticancer Research, 24(2 B), 955-962.