Expression of Caenorhabditis elegans RNA-directed RNA polymerase in transgenic Drosophila melanogaster does not affect morphological development

Guowen Duan, Robert B. Saint, Chris A. Helliwell, Carolyn A. Behm, Peter M. Waterhouse, Karl H.J. Gordon

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Drosophila melanogaster, along with all insects and the vertebrates, lacks an RdRp gene. We created transgenic strains of Drosophila melanogaster in which the rrf-1 or ego-1 RdRp genes from C. elegans were placed under the control of the yeast GAL4 upstream activation sequence. Activation of the gene was performed by crossing these lines to flies carrying the GAL4 transgene under the control of various Drosophila enhancers. RT–PCR confirmed the successful expression of each RdRp gene. The resulting phenotypes indicated that introduction of the RdRp genes had no effect on D. melanogaster morphological development.
Original languageEnglish
Pages (from-to)1121-1128
Number of pages8
JournalTRANSGENIC RESEARCH
Volume19
Issue number6
DOIs
Publication statusPublished - Dec 2010

Keywords

  • MicroRNAs (miRNAs)
  • RNA interference (RNAi)
  • RNA-directed RNA polymerase (RdRp)

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