TY - JOUR
T1 - Expression of Long Non-Coding RNAs in Activated Human Retinal Vascular Endothelial Cells
AU - Barros Ferreira, Lisia
AU - Ashander, Liam M.
AU - Appukuttan, Binoy
AU - Ma, Yuefang
AU - Williams, Keryn A.
AU - Smith, Justine R.
PY - 2023
Y1 - 2023
N2 - Purpose: Retinal endothelial cell activation is a central event in non-infectious posterior uveitis. There is recent interest in long non-coding (lnc)RNA-targeted therapeutics for retinal diseases. We aimed to identify human retinal endothelial cell lncRNAs that might be involved in activation.Methods: Eleven candidate lncRNAs were identified: GAS5, KCNQ1OT1, LINC00294, MALAT1, MEG3, MIR155HG, NEAT1, NORAD, OIP5-AS1, SENCR, TUG1. Expression was assessed by RT-PCR in human retinal endothelial cells, at baseline and following activation with interleukin (IL)-1β and tumor necrosis factor (TNF)-α.Results: IL-1β significantly upregulated MEG3 and SENCR at 4 and 24 hours; LINC00294, NORAD, OIP5-AS1 and TUG1 at 24 hours; and MIR155HG at 4, 24 and 48 hours; but downregulated GAS5 at 24 and 48 hours. TNF-α significantly upregulated KCNQ1OT1, LINC00294, MEG3, NORAD and SENCR at 4 hours; SENCR and TUG1 at 24 hours; and MIR155HG at all time points.Conclusions: Future studies involving manipulation of MIR155HG may be warranted to explore potential therapeutic applications for non-infectious posterior uveitis.
AB - Purpose: Retinal endothelial cell activation is a central event in non-infectious posterior uveitis. There is recent interest in long non-coding (lnc)RNA-targeted therapeutics for retinal diseases. We aimed to identify human retinal endothelial cell lncRNAs that might be involved in activation.Methods: Eleven candidate lncRNAs were identified: GAS5, KCNQ1OT1, LINC00294, MALAT1, MEG3, MIR155HG, NEAT1, NORAD, OIP5-AS1, SENCR, TUG1. Expression was assessed by RT-PCR in human retinal endothelial cells, at baseline and following activation with interleukin (IL)-1β and tumor necrosis factor (TNF)-α.Results: IL-1β significantly upregulated MEG3 and SENCR at 4 and 24 hours; LINC00294, NORAD, OIP5-AS1 and TUG1 at 24 hours; and MIR155HG at 4, 24 and 48 hours; but downregulated GAS5 at 24 and 48 hours. TNF-α significantly upregulated KCNQ1OT1, LINC00294, MEG3, NORAD and SENCR at 4 hours; SENCR and TUG1 at 24 hours; and MIR155HG at all time points.Conclusions: Future studies involving manipulation of MIR155HG may be warranted to explore potential therapeutic applications for non-infectious posterior uveitis.
KW - Cytokine
KW - endothelium
KW - lncRNA
KW - retina
KW - uveitis
UR - http://www.scopus.com/inward/record.url?scp=85139453141&partnerID=8YFLogxK
U2 - 10.1080/09273948.2022.2122512
DO - 10.1080/09273948.2022.2122512
M3 - Article
C2 - 36194865
AN - SCOPUS:85139453141
SN - 0927-3948
VL - 31
SP - 1813
EP - 1818
JO - Ocular Immunology and Inflammation
JF - Ocular Immunology and Inflammation
IS - 9
ER -
