Expression of Long Non-Coding RNAs in Activated Human Retinal Vascular Endothelial Cells

Lisia Barros Ferreira, Liam M. Ashander, Binoy Appukuttan, Yuefang Ma, Keryn A. Williams, Justine R. Smith

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: Retinal endothelial cell activation is a central event in non-infectious posterior uveitis. There is recent interest in long non-coding (lnc)RNA-targeted therapeutics for retinal diseases. We aimed to identify human retinal endothelial cell lncRNAs that might be involved in activation.

Methods: Eleven candidate lncRNAs were identified: GAS5, KCNQ1OT1, LINC00294, MALAT1, MEG3, MIR155HG, NEAT1, NORAD, OIP5-AS1, SENCR, TUG1. Expression was assessed by RT-PCR in human retinal endothelial cells, at baseline and following activation with interleukin (IL)-1β and tumor necrosis factor (TNF)-α.

Results: IL-1β significantly upregulated MEG3 and SENCR at 4 and 24 hours; LINC00294, NORAD, OIP5-AS1 and TUG1 at 24 hours; and MIR155HG at 4, 24 and 48 hours; but downregulated GAS5 at 24 and 48 hours. TNF-α significantly upregulated KCNQ1OT1, LINC00294, MEG3, NORAD and SENCR at 4 hours; SENCR and TUG1 at 24 hours; and MIR155HG at all time points.

Conclusions: Future studies involving manipulation of MIR155HG may be warranted to explore potential therapeutic applications for non-infectious posterior uveitis.

Original languageEnglish
Number of pages7
JournalOcular Immunology and Inflammation
Early online date4 Oct 2022
DOIs
Publication statusE-pub ahead of print - 4 Oct 2022

Keywords

  • Cytokine
  • endothelium
  • lncRNA
  • retina
  • uveitis

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