Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling

Kening Zhao, Mark Bleackley, David Chisanga, Lahiru Gangoda, Pamali Fonseka, Michael Liem, Hina Kalra, Haidar Al Saffar, Shivakumar Keerthikumar, Ching-Seng Ang, Christopher G. Adda, Lanzhou Jiang, Kuok Yap, Ivan K. Poon, Peter Lock, Vincent Bulone, Marilyn Anderson, Suresh Mathivanan

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)
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Abstract

Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1∆ or chs3∆ or the vps23∆chs3∆ double knockout strain were unable to rescue the yeast cells as compared to vps23∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.

Original languageEnglish
Article number305
Number of pages13
JournalCommunications Biology
Volume2
DOIs
Publication statusPublished - 9 Aug 2019
Externally publishedYes

Bibliographical note

Funding Information:
Suresh Mathivanan is supported by Australian Research Council DECRA (DE150101777), Australian Research Council Discovery Project (DP170102312) and Australian Research Council Future Fellowship (FT180100333). Australian Research Council Discovery Project (DP160100309) supports the work of M.B., M.A. and V.B. Electron and optical microscopy presented in this study was performed in the LIMS Bioimaging Facility. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Confocal microscopy was performed in the LIMS BioImaging facility.

Publisher Copyright:
© 2019, The Author(s).

Keywords

  • Extracellular vesicles
  • EVs
  • membranous vesicles
  • cells
  • Endosomal Sorting Complex Required for Transport
  • ESCRT
  • ESCRT machinery
  • morphology
  • antifungal molecules
  • Saccharomyces cerevisiae
  • cell wall remodelling

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