Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

Phikunthiong Kopermsub; Varissaporn Mayen; Steven McInnes; Nicolas Voelcker

doi:10.1109/NANO.2011.6144461

Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

Phikunthiong Kopermsub, Varissaporn Mayen, Steven McInnes, Nicolas Voelcker

Research output: Contribution to conference › Paper › peer-review

1 Citation (Scopus)

Abstract

RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.

Original language	English
Pages	830-832
Number of pages	3
DOIs	https://doi.org/10.1109/NANO.2011.6144461
Publication status	Published - 1 Dec 2011
Event	11th IEEE International Conference on Nanotechnology - Duration: 15 Aug 2011 → …

Conference

Conference	11th IEEE International Conference on Nanotechnology
Period	15/08/11 → …

Keywords

amine-functionalization
porous silicon nanoparticles
siRNA delivery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/NANO.2011.6144461

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title = "Fabrication and characterization of porous silicon nanoparticles for siRNA delivery",

abstract = "RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.",

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author = "Phikunthiong Kopermsub and Varissaporn Mayen and Steven McInnes and Nicolas Voelcker",

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doi = "10.1109/NANO.2011.6144461",

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note = "11th IEEE International Conference on Nanotechnology ; Conference date: 15-08-2011",

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AU - Kopermsub, Phikunthiong

AU - Mayen, Varissaporn

AU - McInnes, Steven

AU - Voelcker, Nicolas

PY - 2011/12/1

Y1 - 2011/12/1

N2 - RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.

AB - RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.

KW - amine-functionalization

KW - porous silicon nanoparticles

KW - siRNA delivery

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Y2 - 15 August 2011

ER -

Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

Abstract

Conference

Keywords

UN SDGs

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