Fabrication and characterization of porous silicon nanoparticles for siRNA delivery

Phikunthiong Kopermsub, Varissaporn Mayen, Steven McInnes, Nicolas Voelcker

    Research output: Contribution to conferencePaper

    1 Citation (Scopus)

    Abstract

    RNA interference (RNAi) using small interfering RNA (siRNA) has been proposed for the therapy of human diseases and in particular cancers. Due to the instability and physicochemical properties of siRNA, the development of siRNA delivery system has attracted much attention. This study aimed at the fabrication of porous silicon nanoparticles (pSi NPs) as carriers for siRNA. pSi NPs possess a high surface area and are biodegradable and biocompatible. pSi NPs with two types of surface chemistry (oxidized and amine-functionalized) were fabricated and characterized. The surface area of pSi NPs was found to be 390 m2/g with a pore width of 9.2 nm. To investigate siRNA loading efficiency of pSi NPs, duplex siRNA was incubated with nanoparticles for 1 hour. The loading capacity of oxidized pSi NPs and amine-functionalized pSi NPs was found to be 1.95 g/mg particles and 2.02 g/mg particles, respectively. Further investigation on cellular uptake and gene silencing of siRNA-loaded pSi nanoparticles will be conducted to provide more details on their possible use for siRNA delivery.

    Original languageEnglish
    Pages830-832
    Number of pages3
    DOIs
    Publication statusPublished - 1 Dec 2011
    Event11th IEEE International Conference on Nanotechnology -
    Duration: 15 Aug 2011 → …

    Conference

    Conference11th IEEE International Conference on Nanotechnology
    Period15/08/11 → …

    Keywords

    • amine-functionalization
    • porous silicon nanoparticles
    • siRNA delivery

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  • Cite this

    Kopermsub, P., Mayen, V., McInnes, S., & Voelcker, N. (2011). Fabrication and characterization of porous silicon nanoparticles for siRNA delivery. 830-832. Paper presented at 11th IEEE International Conference on Nanotechnology, . https://doi.org/10.1109/NANO.2011.6144461