Factors affecting peak height variability for short tandem repeat data

Duncan Taylor, John Buckleton, Jo-Anne Bright

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    In forensic DNA analysis a DNA extract is amplified using polymerase chain reaction (PCR), separated using capillary electrophoresis and the resulting DNA products are detected using fluorescence. Sampling variation occurs when the DNA molecules are aliquotted during the PCR setup stage and this translates to variability in peak heights in the resultant electropherogram or between electropherograms generated from a DNA extract. Beyond the variability caused by sampling variation it has been observed that there are factors in generating the DNA profile that can contribute to the magnitude of variability observed, most notably the number of PCR cycles. In this study we investigate a number of factors in the generation of a DNA profile to determine which contribute to levels of peak height variability.

    Original languageEnglish
    Pages (from-to)126-133
    Number of pages8
    JournalForensic Science International: Genetics
    Volume21
    DOIs
    Publication statusPublished - 1 Mar 2016

    Keywords

    • Peak height variability
    • Stochastic effects
    • STR
    • STRmix

    Fingerprint

    Dive into the research topics of 'Factors affecting peak height variability for short tandem repeat data'. Together they form a unique fingerprint.

    Cite this