Factors influencing RNA yield from placenta tissue

Anya L. Arthurs; Dylan McCullough; Jessica M. Williamson; Tanja Jankovic-Karasoulos; Melanie D. Smith; Claire T. Roberts

doi:10.1016/j.placenta.2023.08.002

Factors influencing RNA yield from placenta tissue

Anya L. Arthurs, Dylan McCullough, Jessica M. Williamson, Tanja Jankovic-Karasoulos, Melanie D. Smith, Claire T. Roberts

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Abstract

High yield and integrity of placental RNA are crucial for placental transcriptomics studies. We assessed the effects of time to placental collection post-delivery; tissue storage, amount and method used for extraction; mode of delivery; and tissue type on total RNA yield. The optimal protocol for RNA extraction from placental tissue includes cryofreezing of the sample upon collection and RNA extraction from 50 mg of tissue using TRIzol reagent. Decidua yielded highest RNA quantity/mg of tissue, followed by villous tissue and the chorion. Comparisons with murine kidney and HEK293T show lower placental RNA yield, likely due to highly dense and heterogeneous tissue make-up and potential high placental nuclease activity.

Original language	English
Pages (from-to)	80-83
Number of pages	4
Journal	PLACENTA
Volume	140
Early online date	3 Aug 2023
DOIs	https://doi.org/10.1016/j.placenta.2023.08.002
Publication status	Published - 7 Sept 2023

Keywords

Extraction
Placenta
RNA
Storage
TRIzol

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Final published versionFinal published version, 1.5 MBLicence: CC BY-NC

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AU - Arthurs, Anya L.

AU - McCullough, Dylan

AU - Williamson, Jessica M.

AU - Jankovic-Karasoulos, Tanja

AU - Smith, Melanie D.

AU - Roberts, Claire T.

PY - 2023/9/7

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AB - High yield and integrity of placental RNA are crucial for placental transcriptomics studies. We assessed the effects of time to placental collection post-delivery; tissue storage, amount and method used for extraction; mode of delivery; and tissue type on total RNA yield. The optimal protocol for RNA extraction from placental tissue includes cryofreezing of the sample upon collection and RNA extraction from 50 mg of tissue using TRIzol reagent. Decidua yielded highest RNA quantity/mg of tissue, followed by villous tissue and the chorion. Comparisons with murine kidney and HEK293T show lower placental RNA yield, likely due to highly dense and heterogeneous tissue make-up and potential high placental nuclease activity.

KW - Extraction

KW - Placenta

KW - RNA

KW - Storage

KW - TRIzol

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Factors influencing RNA yield from placenta tissue

Abstract

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