FAT/CD36 expression alone is insufficient to enhance cellular uptake of oleate

Nicholas S. Eyre, Leslie G. Cleland, Graham Mayrhofer

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Fatty acid translocase (FAT/CD36) is one of several proteins implicated in receptor-mediated uptake of long-chain fatty acids (LCFAs). We have tested whether levels of FAT/CD36 correlate with cellular oleic acid import, using a Tet-Off inducible transfected CHO cell line. Consistent with our previous findings, FAT/CD36 was enriched in lipid raft-derived detergent-resistant membranes (DRMs) that also contained caveolin-1, the marker protein of caveolae. Furthermore in transfected cells, plasma membrane FAT/CD36 co-localized extensively with the lipid raft-enriched ganglioside GM1, and partially with a caveolin-1-EGFP fusion protein. Nevertheless, even at high levels of expression, FAT/CD36 did not affect uptake of oleic acid. We propose that the ability of FAT/CD36 to mediate enhanced uptake of LCFAs is dependent on co-expression of other proteins or factors that are lacking in CHO cells. Crown

Original languageEnglish
Pages (from-to)404-409
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume370
Issue number3
DOIs
Publication statusPublished - 6 Jun 2008
Externally publishedYes

Keywords

  • Caveolae
  • FAT/CD36
  • Lipid rafts
  • Long-chain fatty acid transport
  • Tet-off

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