Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose

Dwaipayan Sen; Ankur Sarkar; Aaron Gosling; Sally Gras; Geoff Stevens; Sandra Kentish; Prashant Bhattacharya; Andrew Barber; Chiranjib Bhattacharjee

doi:10.1016/j.memsci.2011.05.032

Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose

Dwaipayan Sen, Ankur Sarkar, Aaron Gosling, Sally Gras, Geoff Stevens, Sandra Kentish, Prashant Bhattacharya, Andrew Barber, Chiranjib Bhattacharjee

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Present work primarily deals with an exhaustive investigation on the effect of β-galactosidase (EC 3.2.1.23) immobilization on polymeric polyether sulfone, cellulose triacetate and thin film composite polyamide membrane to produce galacto-oligosaccharides from lactose. Fouling is one of the key issues that control any membrane separation process to obtain the desired product. Especially, this issue with membrane becomes multiplied after making any attachment of immobilization chemicals on its surface, i.e. in case of enzymatic membrane reactor. Present work thus aims to identify the insights of carbohydrate interactions with the membrane surface after immobilization and how far it controls the production of galacto-oligosaccharides in this membrane reactor.

Original language	English
Pages (from-to)	471-478
Number of pages	8
Journal	Journal of Membrane Science
Volume	378
Issue number	1-2
DOIs	https://doi.org/10.1016/j.memsci.2011.05.032
Publication status	Published - 15 Aug 2011

Keywords

Carbohydrates
Galacto-oligosaccharides
Immobilization
Lactose
Polymeric membrane

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10.1016/j.memsci.2011.05.032

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title = "Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose",

abstract = "Present work primarily deals with an exhaustive investigation on the effect of β-galactosidase (EC 3.2.1.23) immobilization on polymeric polyether sulfone, cellulose triacetate and thin film composite polyamide membrane to produce galacto-oligosaccharides from lactose. Fouling is one of the key issues that control any membrane separation process to obtain the desired product. Especially, this issue with membrane becomes multiplied after making any attachment of immobilization chemicals on its surface, i.e. in case of enzymatic membrane reactor. Present work thus aims to identify the insights of carbohydrate interactions with the membrane surface after immobilization and how far it controls the production of galacto-oligosaccharides in this membrane reactor.",

keywords = "Carbohydrates, Galacto-oligosaccharides, Immobilization, Lactose, Polymeric membrane",

author = "Dwaipayan Sen and Ankur Sarkar and Aaron Gosling and Sally Gras and Geoff Stevens and Sandra Kentish and Prashant Bhattacharya and Andrew Barber and Chiranjib Bhattacharjee",

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Sen, D, Sarkar, A, Gosling, A, Gras, S, Stevens, G, Kentish, S, Bhattacharya, P, Barber, A & Bhattacharjee, C 2011, 'Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose', Journal of Membrane Science, vol. 378, no. 1-2, pp. 471-478. https://doi.org/10.1016/j.memsci.2011.05.032

TY - JOUR

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AU - Sen, Dwaipayan

AU - Sarkar, Ankur

AU - Gosling, Aaron

AU - Gras, Sally

AU - Stevens, Geoff

AU - Kentish, Sandra

AU - Bhattacharya, Prashant

AU - Barber, Andrew

AU - Bhattacharjee, Chiranjib

PY - 2011/8/15

Y1 - 2011/8/15

N2 - Present work primarily deals with an exhaustive investigation on the effect of β-galactosidase (EC 3.2.1.23) immobilization on polymeric polyether sulfone, cellulose triacetate and thin film composite polyamide membrane to produce galacto-oligosaccharides from lactose. Fouling is one of the key issues that control any membrane separation process to obtain the desired product. Especially, this issue with membrane becomes multiplied after making any attachment of immobilization chemicals on its surface, i.e. in case of enzymatic membrane reactor. Present work thus aims to identify the insights of carbohydrate interactions with the membrane surface after immobilization and how far it controls the production of galacto-oligosaccharides in this membrane reactor.

AB - Present work primarily deals with an exhaustive investigation on the effect of β-galactosidase (EC 3.2.1.23) immobilization on polymeric polyether sulfone, cellulose triacetate and thin film composite polyamide membrane to produce galacto-oligosaccharides from lactose. Fouling is one of the key issues that control any membrane separation process to obtain the desired product. Especially, this issue with membrane becomes multiplied after making any attachment of immobilization chemicals on its surface, i.e. in case of enzymatic membrane reactor. Present work thus aims to identify the insights of carbohydrate interactions with the membrane surface after immobilization and how far it controls the production of galacto-oligosaccharides in this membrane reactor.

KW - Carbohydrates

KW - Galacto-oligosaccharides

KW - Immobilization

KW - Lactose

KW - Polymeric membrane

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DO - 10.1016/j.memsci.2011.05.032

M3 - Article

SN - 0376-7388

VL - 378

SP - 471

EP - 478

JO - Journal of Membrane Science

JF - Journal of Membrane Science

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ER -

Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose

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Keywords

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