Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose

Dwaipayan Sen, Ankur Sarkar, Aaron Gosling, Sally Gras, Geoff Stevens, Sandra Kentish, Prashant Bhattacharya, Andrew Barber, Chiranjib Bhattacharjee

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    Present work primarily deals with an exhaustive investigation on the effect of β-galactosidase (EC 3.2.1.23) immobilization on polymeric polyether sulfone, cellulose triacetate and thin film composite polyamide membrane to produce galacto-oligosaccharides from lactose. Fouling is one of the key issues that control any membrane separation process to obtain the desired product. Especially, this issue with membrane becomes multiplied after making any attachment of immobilization chemicals on its surface, i.e. in case of enzymatic membrane reactor. Present work thus aims to identify the insights of carbohydrate interactions with the membrane surface after immobilization and how far it controls the production of galacto-oligosaccharides in this membrane reactor.

    Original languageEnglish
    Pages (from-to)471-478
    Number of pages8
    JournalJournal of Membrane Science
    Volume378
    Issue number1-2
    DOIs
    Publication statusPublished - 15 Aug 2011

    Keywords

    • Carbohydrates
    • Galacto-oligosaccharides
    • Immobilization
    • Lactose
    • Polymeric membrane

    Fingerprint Dive into the research topics of 'Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose'. Together they form a unique fingerprint.

  • Cite this

    Sen, D., Sarkar, A., Gosling, A., Gras, S., Stevens, G., Kentish, S., Bhattacharya, P., Barber, A., & Bhattacharjee, C. (2011). Feasibility study of enzyme immobilization on polymeric membrane: A case study with enzymatically galacto-oligosaccharides production from lactose. Journal of Membrane Science, 378(1-2), 471-478. https://doi.org/10.1016/j.memsci.2011.05.032