Fifteen microsatellite loci for use in non-invasive sampling studies of antilopine wallaroo (Macropus antilopinus)

Jessica Wadley, Jeremy Austin, Michael Gardner, Damien Fordham

    Research output: Contribution to journalArticle

    Abstract

    A set of 15 microsatellite loci was optimised for multilocus genotyping of non-invasively collected samples of Macropus antilopinus (antilopine wallaroo). Primers were combined in three PCR multiplexes in order to increase the quality of genotypes from scat samples and to allow for replication. In a screen of 104 scat samples from two populations in north-eastern Australia, three loci were found to be monomorphic while the remaining 12 loci had 2-10 alleles. Genotype frequencies for all 12 microsatellite loci from the two populations did not differ significantly from Hardy-Weinberg equilibrium, and there was no evidence of linkage disequilibrium. These informative markers are specifically designed for non-invasive samples and will be used to assess population structure and conservation genetics of this species in the future.

    Original languageEnglish
    Pages (from-to)399-401
    Number of pages3
    JournalAustralian Journal of Zoology
    Volume61
    Issue number5
    DOIs
    Publication statusPublished - 2013

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