Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo (Macropus antilopinus)

Jessica Wadley; Jeremy Austin; Michael Gardner; Damien Fordham

doi:10.1071/ZO13074

Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo (Macropus antilopinus)

Jessica Wadley, Jeremy Austin, Michael Gardner, Damien Fordham

Research output: Contribution to journal › Article › peer-review

Abstract

A set of 15 microsatellite loci was optimised for multilocus genotyping of non-invasively collected samples of Macropus antilopinus (antilopine wallaroo). Primers were combined in three PCR multiplexes in order to increase the quality of genotypes from scat samples and to allow for replication. In a screen of 104 scat samples from two populations in north-eastern Australia, three loci were found to be monomorphic while the remaining 12 loci had 2-10 alleles. Genotype frequencies for all 12 microsatellite loci from the two populations did not differ significantly from Hardy-Weinberg equilibrium, and there was no evidence of linkage disequilibrium. These informative markers are specifically designed for non-invasive samples and will be used to assess population structure and conservation genetics of this species in the future.

Original language	English
Pages (from-to)	399-401
Number of pages	3
Journal	Australian Journal of Zoology
Volume	61
Issue number	5
DOIs	https://doi.org/10.1071/ZO13074
Publication status	Published - 2013

Keywords

faeces
kangaroo
macropod
non-invasive genotyping
population genetics.

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10.1071/ZO13074

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title = "Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo (Macropus antilopinus)",

abstract = "A set of 15 microsatellite loci was optimised for multilocus genotyping of non-invasively collected samples of Macropus antilopinus (antilopine wallaroo). Primers were combined in three PCR multiplexes in order to increase the quality of genotypes from scat samples and to allow for replication. In a screen of 104 scat samples from two populations in north-eastern Australia, three loci were found to be monomorphic while the remaining 12 loci had 2-10 alleles. Genotype frequencies for all 12 microsatellite loci from the two populations did not differ significantly from Hardy-Weinberg equilibrium, and there was no evidence of linkage disequilibrium. These informative markers are specifically designed for non-invasive samples and will be used to assess population structure and conservation genetics of this species in the future.",

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AU - Austin, Jeremy

AU - Gardner, Michael

AU - Fordham, Damien

PY - 2013

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Fifteen microsatellite loci for use in non-invasive sampling studies of the antilopine wallaroo (Macropus antilopinus)

Abstract

Keywords

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